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Which set of the following events occurs during the elongation step of translation?
P. Attachment of mRNA with the smaller subunit of ribosome
Q. Loading of correct aminoacyl-tRNA into the A site
R. Formation of a peptide bond between the amino acyl-tRNA in the A site and the peptide chain that is attached to the peptidyl-tRNA in the P site
S. Dissociation of the ribosomal subunits
T. Translocation of peptidyl-tRNA from the A site to the P site of the ribosome
  • a)
    P, Q and R
  • b)
    P, Q and T
  • c)
    Q, R and T
  • d)
    R, S and T
Correct answer is option 'C'. Can you explain this answer?
Verified Answer
Which set of the following events occurs during the elongation step of...
During chain elongation, each additional amino acid is added to the nascent polypeptide chain in a three-step microcycle. The steps in this microcycle are (1) positioning the correct aminoacyltRNA in the A site of the ribosome, (2) forming the peptide bond and (3) shifting the mRNA by one codon relative to the ribosome On the other hand, non-competitive inhibitors do NOT bind to the active site of the enzyme and do not resemble the substrate. Therefore, addition of more substrate cannot eliminate the effect of the inhibitor. As a result, there is always a fixed amount of enzyme inactive in non-competitive inhibition. As you recall, when you change the amount of enzyme, you change the Vmax (from last lecture), so in the presence of a non-competitive inhibitor, the Vmax decreases.
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Most Upvoted Answer
Which set of the following events occurs during the elongation step of...
Key Points:
- Elongation is the second step of translation where the polypeptide chain is synthesized.
- The events that occur during elongation are:
1. Loading of correct aminoacyl-tRNA into the A site
2. Formation of a peptide bond between the aminoacyl-tRNA in the A site and the peptidyl-tRNA in the P site
3. Translocation of peptidyl-tRNA from the A site to the P site of the ribosome

Explanation:
During the elongation step of translation, the ribosome moves along the mRNA template and adds amino acids to the growing polypeptide chain. Several key events occur during this process.

1. Loading of correct aminoacyl-tRNA into the A site:
- The smaller subunit of the ribosome attaches to the mRNA molecule, and the mRNA is threaded through the ribosome until the start codon (AUG) is reached.
- The anticodon of the incoming aminoacyl-tRNA base pairs with the codon on the mRNA in the A site of the ribosome.
- This ensures that the correct amino acid is added to the growing polypeptide chain.

2. Formation of a peptide bond:
- The ribosome catalyzes the formation of a peptide bond between the amino acid of the incoming aminoacyl-tRNA in the A site and the peptide chain attached to the peptidyl-tRNA in the P site.
- This forms a dipeptide and releases the peptidyl-tRNA from the P site.

3. Translocation of peptidyl-tRNA:
- After the peptide bond formation, the ribosome translocates along the mRNA molecule by one codon.
- This movement shifts the peptidyl-tRNA from the A site to the P site, and the A site becomes available for the next aminoacyl-tRNA to enter.
- The empty tRNA in the E site is released from the ribosome.

Inference:
From the given options, the correct set of events that occur during the elongation step of translation is Q, R, and T.
- The loading of correct aminoacyl-tRNA into the A site (Q) ensures the addition of the correct amino acid to the growing polypeptide chain.
- The formation of a peptide bond between the aminoacyl-tRNA in the A site and the peptidyl-tRNA in the P site (R) results in the synthesis of a dipeptide.
- The translocation of peptidyl-tRNA from the A site to the P site of the ribosome (T) allows the ribosome to move along the mRNA and continue synthesizing the polypeptide chain.
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Which set of the following events occurs during the elongation step of translation?P. Attachment of mRNA with the smaller subunit of ribosomeQ. Loading of correct aminoacyl-tRNA into the A siteR. Formation of a peptide bond between the amino acyl-tRNA in the A site and the peptide chain that is attached to the peptidyl-tRNA in the P siteS. Dissociation of the ribosomal subunitsT. Translocation of peptidyl-tRNA from the A site to the P site of the ribosomea)P, Q and Rb)P, Q and Tc)Q, R and Td)R, S and TCorrect answer is option 'C'. Can you explain this answer?
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