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c DNA library is based on
- a)DNA dependent DNA polymerase
- b)RNA dependent DNA polymerase
- c)RNA dependent RNA polymerase
- d)DNA dependent RNA polymerase
Correct answer is option 'B'. Can you explain this answer?
Most Upvoted Answer
c DNA library is based ona)DNA dependent DNA polymeraseb)RNA dependent...
Explanation:
A cDNA library is a collection of cDNA clones that represent the genes expressed in a particular cell type or tissue. It is used for gene discovery and studying gene expression. The library is made by converting mRNA into cDNA using the enzyme reverse transcriptase, and then cloning the cDNA into a plasmid vector.
RNA dependent DNA polymerase:
RNA-dependent DNA polymerase is also called reverse transcriptase. It is an enzyme that catalyzes the formation of DNA from an RNA template. It is widely used in molecular biology for the synthesis of cDNA from RNA templates.
cDNA library:
A cDNA library is made by converting mRNA into cDNA using reverse transcriptase, and then cloning the cDNA into a plasmid vector. The resulting library contains only cDNAs, which are DNA copies of the expressed mRNA molecules. The library is used to study gene expression and for gene discovery.
Why RNA dependent DNA polymerase is used in cDNA library preparation:
Reverse transcriptase is used in cDNA library preparation because it can synthesize a complementary DNA strand from an RNA template. This allows the synthesis of cDNA from the mRNA present in a particular cell type or tissue. The cDNA can then be cloned into a plasmid vector to create a cDNA library.
Conclusion:
In conclusion, a cDNA library is made by using RNA-dependent DNA polymerase (reverse transcriptase) to convert mRNA into cDNA, which is then cloned into a plasmid vector. This allows the study of gene expression and gene discovery in a particular cell type or tissue.
A cDNA library is a collection of cDNA clones that represent the genes expressed in a particular cell type or tissue. It is used for gene discovery and studying gene expression. The library is made by converting mRNA into cDNA using the enzyme reverse transcriptase, and then cloning the cDNA into a plasmid vector.
RNA dependent DNA polymerase:
RNA-dependent DNA polymerase is also called reverse transcriptase. It is an enzyme that catalyzes the formation of DNA from an RNA template. It is widely used in molecular biology for the synthesis of cDNA from RNA templates.
cDNA library:
A cDNA library is made by converting mRNA into cDNA using reverse transcriptase, and then cloning the cDNA into a plasmid vector. The resulting library contains only cDNAs, which are DNA copies of the expressed mRNA molecules. The library is used to study gene expression and for gene discovery.
Why RNA dependent DNA polymerase is used in cDNA library preparation:
Reverse transcriptase is used in cDNA library preparation because it can synthesize a complementary DNA strand from an RNA template. This allows the synthesis of cDNA from the mRNA present in a particular cell type or tissue. The cDNA can then be cloned into a plasmid vector to create a cDNA library.
Conclusion:
In conclusion, a cDNA library is made by using RNA-dependent DNA polymerase (reverse transcriptase) to convert mRNA into cDNA, which is then cloned into a plasmid vector. This allows the study of gene expression and gene discovery in a particular cell type or tissue.
Community Answer
c DNA library is based ona)DNA dependent DNA polymeraseb)RNA dependent...
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c DNA library is based ona)DNA dependent DNA polymeraseb)RNA dependent DNA polymerasec)RNA dependent RNA polymerase d)DNA dependent RNA polymeraseCorrect answer is option 'B'. Can you explain this answer?
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c DNA library is based ona)DNA dependent DNA polymeraseb)RNA dependent DNA polymerasec)RNA dependent RNA polymerase d)DNA dependent RNA polymeraseCorrect answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about c DNA library is based ona)DNA dependent DNA polymeraseb)RNA dependent DNA polymerasec)RNA dependent RNA polymerase d)DNA dependent RNA polymeraseCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for c DNA library is based ona)DNA dependent DNA polymeraseb)RNA dependent DNA polymerasec)RNA dependent RNA polymerase d)DNA dependent RNA polymeraseCorrect answer is option 'B'. Can you explain this answer?.
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