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If EcoR1 cuts the dsDNA fragment at four places, then what is the ratio of number of hydrogen bonds present at those locations before cleavage by EcoR1 and number of hydrogen bonds cleaved by EcoR1 at that location respectively? 1)8:14 2) 7:4 3) 8:2 4)6:2?
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If EcoR1 cuts the dsDNA fragment at four places, then what is the rati...
Ratio of Hydrogen Bonds Before and After EcoR1 Cleavage
Introduction: EcoR1 is a restriction endonuclease that cuts DNA at specific recognition sequences, resulting in fragments of varying lengths. The number of hydrogen bonds present at the cleavage site before and after EcoR1 cleavage is important to understand for various molecular biology applications.
Method: To determine the ratio of hydrogen bonds before and after EcoR1 cleavage, we need to know the recognition sequence of EcoR1 and the number of hydrogen bonds present at the cleavage site.
Result: EcoR1 recognizes the sequence GAATTC and cleaves the DNA between the G and the A, producing fragments with overhangs. The number of hydrogen bonds present at the cleavage site before and after EcoR1 cleavage is as follows:
Before cleavage:
- Between G and C: 3 hydrogen bonds
- Between A and T: 2 hydrogen bonds
- Total: 5 hydrogen bonds
After cleavage:
- Between G and A: 2 hydrogen bonds
- Between T and T: 2 hydrogen bonds
- Total: 4 hydrogen bonds
Therefore, the ratio of hydrogen bonds before and after EcoR1 cleavage is:
5:4
Conclusion: The ratio of hydrogen bonds before and after EcoR1 cleavage is 5:4. This information is important for various molecular biology applications, such as designing PCR primers and cloning DNA fragments.
Introduction: EcoR1 is a restriction endonuclease that cuts DNA at specific recognition sequences, resulting in fragments of varying lengths. The number of hydrogen bonds present at the cleavage site before and after EcoR1 cleavage is important to understand for various molecular biology applications.
Method: To determine the ratio of hydrogen bonds before and after EcoR1 cleavage, we need to know the recognition sequence of EcoR1 and the number of hydrogen bonds present at the cleavage site.
Result: EcoR1 recognizes the sequence GAATTC and cleaves the DNA between the G and the A, producing fragments with overhangs. The number of hydrogen bonds present at the cleavage site before and after EcoR1 cleavage is as follows:
Before cleavage:
- Between G and C: 3 hydrogen bonds
- Between A and T: 2 hydrogen bonds
- Total: 5 hydrogen bonds
After cleavage:
- Between G and A: 2 hydrogen bonds
- Between T and T: 2 hydrogen bonds
- Total: 4 hydrogen bonds
Therefore, the ratio of hydrogen bonds before and after EcoR1 cleavage is:
5:4
Conclusion: The ratio of hydrogen bonds before and after EcoR1 cleavage is 5:4. This information is important for various molecular biology applications, such as designing PCR primers and cloning DNA fragments.
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If EcoR1 cuts the dsDNA fragment at four places, then what is the rati...
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If EcoR1 cuts the dsDNA fragment at four places, then what is the ratio of number of hydrogen bonds present at those locations before cleavage by EcoR1 and number of hydrogen bonds cleaved by EcoR1 at that location respectively? 1)8:14 2) 7:4 3) 8:2 4)6:2?
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If EcoR1 cuts the dsDNA fragment at four places, then what is the ratio of number of hydrogen bonds present at those locations before cleavage by EcoR1 and number of hydrogen bonds cleaved by EcoR1 at that location respectively? 1)8:14 2) 7:4 3) 8:2 4)6:2? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If EcoR1 cuts the dsDNA fragment at four places, then what is the ratio of number of hydrogen bonds present at those locations before cleavage by EcoR1 and number of hydrogen bonds cleaved by EcoR1 at that location respectively? 1)8:14 2) 7:4 3) 8:2 4)6:2? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If EcoR1 cuts the dsDNA fragment at four places, then what is the ratio of number of hydrogen bonds present at those locations before cleavage by EcoR1 and number of hydrogen bonds cleaved by EcoR1 at that location respectively? 1)8:14 2) 7:4 3) 8:2 4)6:2?.
If EcoR1 cuts the dsDNA fragment at four places, then what is the ratio of number of hydrogen bonds present at those locations before cleavage by EcoR1 and number of hydrogen bonds cleaved by EcoR1 at that location respectively? 1)8:14 2) 7:4 3) 8:2 4)6:2? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If EcoR1 cuts the dsDNA fragment at four places, then what is the ratio of number of hydrogen bonds present at those locations before cleavage by EcoR1 and number of hydrogen bonds cleaved by EcoR1 at that location respectively? 1)8:14 2) 7:4 3) 8:2 4)6:2? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If EcoR1 cuts the dsDNA fragment at four places, then what is the ratio of number of hydrogen bonds present at those locations before cleavage by EcoR1 and number of hydrogen bonds cleaved by EcoR1 at that location respectively? 1)8:14 2) 7:4 3) 8:2 4)6:2?.
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