Which one of the following is a template independent enzyme? 1)DNA polymerase 2)RNA polymerase 3)poly A polymerase 4)reverse transcriptase The given answer is3?

Question

Answer

Poly A Polymerase is the answer. It calayses the template independent addition of AMP from ATP to the 3' end of RNA.

Answer

Poly(A) polymerase, the enzyme at the heart of the polyadenylation machinery, is a template-independent RNA polymerase which specifically incorporates ATP at the 3′ end of mRNA. ... The C-terminal domain unexpectedly folds into a compact domain reminiscent of the RNA-recognition motif fold.

Answer

**Template-Independent Enzyme: Poly A Polymerase**

Poly A polymerase is the correct answer as it is a template-independent enzyme. To understand why, let's first define what a template-independent enzyme is.

A template-independent enzyme is an enzyme that does not require a template or a pre-existing nucleic acid strand to synthesize a new strand of nucleic acid. These enzymes can add nucleotides to the growing chain without the need for a complementary template strand.

**Explanation:**

1) DNA Polymerase:
DNA polymerase is the enzyme responsible for synthesizing new DNA strands during DNA replication. It requires a template strand to guide the synthesis of the complementary strand. It reads the template strand and adds complementary nucleotides to the growing chain.

2) RNA Polymerase:
RNA polymerase is the enzyme responsible for synthesizing RNA strands during transcription. It also requires a template DNA strand to guide the synthesis of the complementary RNA strand. It reads the template DNA strand and adds complementary ribonucleotides to the growing RNA chain.

3) Poly A Polymerase:
Poly A polymerase is an enzyme that adds a string of adenine nucleotides (poly A tail) to the 3' end of an RNA molecule. This enzyme does not require a template strand to add the adenine nucleotides. It simply adds the adenine nucleotides one by one to the growing RNA chain.

4) Reverse Transcriptase:
Reverse transcriptase is an enzyme found in retroviruses that can synthesize a DNA strand from an RNA template. It requires an RNA template to synthesize the complementary DNA strand.

**Conclusion:**
Among the given options, poly A polymerase is the only template-independent enzyme. It can add a string of adenine nucleotides to the 3' end of an RNA molecule without the need for a template strand. DNA polymerase, RNA polymerase, and reverse transcriptase all require template strands for their respective synthesis processes.

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