Identify the wrong statement with regard to restriction enzymes.a)They...
The wrong statement is:
Each restriction enzyme functions by inspecting the length of a DNA sequence.
Explanation:
Restriction enzymes are proteins that recognize and cut specific DNA sequences, not by inspecting the length of the sequence. They recognize specific palindromic sequences in the DNA (statement 4) and cut at precise positions within those sequences. These enzymes are useful in genetic engineering (statement 1) because they enable researchers to cut and manipulate DNA fragments in a controlled manner. When restriction enzymes create "sticky ends" (overhanging single-stranded DNA), these sticky ends can be joined together using DNA ligases (statement 2), allowing for the construction of recombinant DNA molecules.
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Identify the wrong statement with regard to restriction enzymes.a)They...
Explanation:
Restriction enzymes are enzymes that cut DNA at specific recognition sites. They are essential tools in genetic engineering and are widely used in the construction of recombinant DNA molecules.
a) Correct statement: They are useful in genetic engineering.
Restriction enzymes are commonly used in genetic engineering to cut DNA at specific sites and create recombinant DNA molecules that can be used for a variety of purposes, such as gene therapy, cloning, and genetic modification of organisms.
b) Incorrect statement: Sticky ends can be joined by using DNA ligases.
Sticky ends are the single-stranded overhangs that result from the action of restriction enzymes on DNA. These ends can be joined to other complementary ends using DNA ligases. Therefore, the statement is incorrect because the sticky ends are joined by DNA ligases, not the restriction enzymes.
c) Correct statement: Each restriction enzyme functions by inspecting the length of a DNA sequence.
Each restriction enzyme recognizes a specific DNA sequence, usually a palindromic sequence, and cuts the DNA at a specific site within that sequence. The length of the DNA sequence recognized by the enzyme can vary from 4 to 8 base pairs.
d) Correct statement: They cut the strand of DNA at palindromic sites.
Restriction enzymes cut the DNA strand at specific recognition sites, which are often palindromic sequences. Palindromic sequences are sequences that read the same way in both directions. The recognition site of a restriction enzyme is usually a palindromic sequence, and the enzyme cuts the DNA strand at a specific site within that sequence.
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