Directions : In the following questions a statement of assertion (A) ...
In Agarose gel electrophoresis, DNAs fragments cut by restriction enzymes can be arranged according to their sizes.
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Directions : In the following questions a statement of assertion (A) ...
**Assertion (A) :** Agarose gel electrophoresis is used to check the progression of a restriction enzyme digestion.
**Reason (R) :** Restriction enzyme digestions are performed by incubating purified DNA molecules with restriction enzymes.
**Explanation:**
Agarose gel electrophoresis is a widely used technique in molecular biology to separate and analyze DNA fragments based on their size. It is commonly used to check the progression of a restriction enzyme digestion.
**Agarose gel electrophoresis:**
- Agarose gel electrophoresis is a technique used to separate DNA fragments based on their size.
- Agarose, a polysaccharide derived from seaweed, is used to create a gel matrix.
- The gel is placed in an electrophoresis chamber, and an electric current is applied.
- DNA samples are loaded into wells at one end of the gel, and the current causes the DNA fragments to migrate through the gel matrix.
- Smaller fragments move faster and migrate further, while larger fragments move slower and migrate less.
- After electrophoresis, the gel is stained with a dye that binds to DNA, allowing the DNA fragments to be visualized.
**Restriction enzyme digestions:**
- Restriction enzymes, also known as restriction endonucleases, are enzymes that recognize specific DNA sequences and cleave the DNA at or near these sequences.
- Restriction enzyme digestions are performed by incubating purified DNA molecules with restriction enzymes.
- The enzymes cut the DNA at specific sites, resulting in the generation of DNA fragments of different sizes.
- These fragments can be subsequently analyzed using agarose gel electrophoresis to determine their sizes.
**Explanation of the Assertion and Reason:**
- The assertion states that agarose gel electrophoresis is used to check the progression of a restriction enzyme digestion.
- This is true because agarose gel electrophoresis allows us to separate and visualize the DNA fragments generated by restriction enzyme digestions.
- The reason states that restriction enzyme digestions are performed by incubating purified DNA molecules with restriction enzymes.
- This is also true as restriction enzymes cleave DNA molecules at specific sites, resulting in the generation of DNA fragments.
**Conclusion:**
- Both the assertion and the reason are true.
- However, the reason alone does not provide a complete explanation for the assertion.
- The reason explains the process of restriction enzyme digestions but does not directly explain why agarose gel electrophoresis is used to check the progression of these digestions.
- Therefore, option B is the correct choice.
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