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Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.
i. Hybridisation using labelled VNTR probe
ii. Isolation of DNA
iii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nylon
iv. Detection of hybridised DNA fragments by autoradiograph
v. Separation of DNA fragments by electrophoresis
vi. Digestion of DNA by restriction endonucleases
- a)i, v, vi, ii, iv and iii
- b)vi, v, vi, ii, i and iii
- c)ii, v, vi, iii, i and iv
- d)ii, vi, v, iii, i and iv
Correct answer is option 'D'. Can you explain this answer?
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Select the correct sequence of steps in DNA finger printing involving...
The correct sequence of steps in DNA fingerprinting involving Southern blot hybridization using VNTR (Variable Number Tandem Repeat) is as follows:
i. Isolation of DNA
ii. Digestion of DNA by restriction endonucleases
iii. Separation of DNA fragments by electrophoresis
iv. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nylon
v. Hybridization using a labelled VNTR probe
vi. Detection of hybridized DNA fragments by autoradiography
Explanation of the steps:
i. Isolation of DNA: DNA is extracted from the sample containing the target DNA, such as blood or tissue.
ii. Digestion of DNA by restriction endonucleases: Restriction enzymes are used to cut the DNA into smaller fragments at specific recognition sites. This step generates a mixture of DNA fragments of varying lengths.
iii. Separation of DNA fragments by electrophoresis: The DNA fragments are loaded onto an agarose gel and subjected to electrophoresis. The negatively charged DNA fragments move towards the positive electrode, and the smaller fragments migrate faster than the larger ones. This separation results in a pattern of bands on the gel, representing the different DNA fragment sizes.
iv. Transferring (blotting) of DNA fragments to synthetic membranes: After electrophoresis, the DNA fragments are transferred from the agarose gel to a synthetic membrane, such as nitrocellulose or nylon, by a process called blotting. This transfer allows for better accessibility of the DNA fragments for subsequent hybridization steps.
v. Hybridization using a labelled VNTR probe: A VNTR probe, which is a short DNA sequence that is complementary to the VNTR region of interest, is used. The probe is labelled with a radioactive or fluorescent marker for detection. The probe is incubated with the membrane, allowing it to hybridize (bind) to the complementary VNTR sequences on the membrane.
vi. Detection of hybridized DNA fragments by autoradiography: After hybridization, the membrane is washed to remove any unbound probe. In the case of radioactive labelling, an autoradiograph is then created by placing the membrane on an X-ray film or a phosphor screen. The radioactive emissions from the probe expose the film or screen, resulting in a pattern of dark bands corresponding to the hybridized DNA fragments.
Therefore, the correct sequence of steps in DNA fingerprinting involving Southern blot hybridization using VNTR is ii, vi, v, iv, i, and iii.
i. Isolation of DNA
ii. Digestion of DNA by restriction endonucleases
iii. Separation of DNA fragments by electrophoresis
iv. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nylon
v. Hybridization using a labelled VNTR probe
vi. Detection of hybridized DNA fragments by autoradiography
Explanation of the steps:
i. Isolation of DNA: DNA is extracted from the sample containing the target DNA, such as blood or tissue.
ii. Digestion of DNA by restriction endonucleases: Restriction enzymes are used to cut the DNA into smaller fragments at specific recognition sites. This step generates a mixture of DNA fragments of varying lengths.
iii. Separation of DNA fragments by electrophoresis: The DNA fragments are loaded onto an agarose gel and subjected to electrophoresis. The negatively charged DNA fragments move towards the positive electrode, and the smaller fragments migrate faster than the larger ones. This separation results in a pattern of bands on the gel, representing the different DNA fragment sizes.
iv. Transferring (blotting) of DNA fragments to synthetic membranes: After electrophoresis, the DNA fragments are transferred from the agarose gel to a synthetic membrane, such as nitrocellulose or nylon, by a process called blotting. This transfer allows for better accessibility of the DNA fragments for subsequent hybridization steps.
v. Hybridization using a labelled VNTR probe: A VNTR probe, which is a short DNA sequence that is complementary to the VNTR region of interest, is used. The probe is labelled with a radioactive or fluorescent marker for detection. The probe is incubated with the membrane, allowing it to hybridize (bind) to the complementary VNTR sequences on the membrane.
vi. Detection of hybridized DNA fragments by autoradiography: After hybridization, the membrane is washed to remove any unbound probe. In the case of radioactive labelling, an autoradiograph is then created by placing the membrane on an X-ray film or a phosphor screen. The radioactive emissions from the probe expose the film or screen, resulting in a pattern of dark bands corresponding to the hybridized DNA fragments.
Therefore, the correct sequence of steps in DNA fingerprinting involving Southern blot hybridization using VNTR is ii, vi, v, iv, i, and iii.
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Select the correct sequence of steps in DNA finger printing involving...
The correct sequence is
i. Isolation of DNA
ii. Digestion of DNA by restriction endonucleases
iii. Separation of DNA fragments by electrophoresis
iv. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nylon
v. Hybridisation using labelled VNTR probe
vi. Detection of hybridised DNA fragments by autoradiograph
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Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer?
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Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer?.
Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer?.
Solutions for Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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Here you can find the meaning of Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of
Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer?, a detailed solution for Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? has been provided alongside types of Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice Select the correct sequence of steps in DNA finger printing involving southern blot hybridisation using VNTR.i. Hybridisation using labelled VNTR probeii. Isolation of DNAiii. Transferring (blotting) of DNA fragments to synthetic membranes, such as nitrocellulose or nyloniv. Detection of hybridised DNA fragments by autoradiographv. Separation of DNA fragments by electrophoresisvi. Digestion of DNA by restriction endonucleasesa)i, v, vi, ii, iv and iiib)vi, v, vi, ii, i and iiic)ii, v, vi, iii, i and ivd)ii, vi, v, iii, i and ivCorrect answer is option 'D'. Can you explain this answer? tests, examples and also practice NEET tests.
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