Insertion of recombinant DNA within the gene encoding for β&ndash...
Insertional inactivation is the process in which the gene is inactivated due to the insertion of other genes within it or within its coding sequence. This leads to loss of function of that specific gene. Thus, insertion of recombinant DNA within the gene encoding for β–galactosidase will lead to insertional inactivation.
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Insertion of recombinant DNA within the gene encoding for β&ndash...
A specific protein can lead to the production of a modified protein with altered properties. This process is known as gene insertion or genetic engineering.
To insert the recombinant DNA into the gene encoding for a specific protein, several steps need to be followed:
1. Isolate the gene: The gene encoding for the specific protein needs to be isolated from the organism's DNA. This can be done using various molecular biology techniques such as polymerase chain reaction (PCR) or DNA sequencing.
2. Generate recombinant DNA: The recombinant DNA is created by inserting the desired gene into a vector, which is typically a plasmid or a viral genome. The vector acts as a carrier to introduce the recombinant DNA into the target organism.
3. Transformation: The recombinant DNA is introduced into the target organism's cells using a process called transformation. This can be done by electroporation, where an electric field is applied to the cells, or by using specialized viruses called viral vectors.
4. Integration of recombinant DNA: Once inside the target organism's cells, the recombinant DNA integrates into the host genome. This can occur through various mechanisms such as homologous recombination or non-homologous end joining.
5. Expression of modified protein: After integration, the recombinant DNA is transcribed into mRNA and then translated into the modified protein. The modified protein may have altered properties compared to the original protein due to the changes introduced by the recombinant DNA.
Overall, the insertion of recombinant DNA into the gene encoding for a specific protein allows for the production of a modified protein with desired properties. This technique has numerous applications in various fields such as medicine, agriculture, and biotechnology.
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