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In the process of insertional inactivation
- a)A recombinant DNA is inserted within the coding sequence of enzyme -galactosidase, resulting in inactivation of the enzyme
- b)A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmid
- c)A recombinat DNA is inserted within the recongnition site for EcoRl
- d)None of the above
Correct answer is option 'A'. Can you explain this answer?
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In the process of insertional inactivationa)A recombinant DNA is inser...
A recombinant DNA is inserted within the coding sequence of enzyme -galactosidase, resulting in inactivation of the enzyme
Alternative selectable markers have been developed which differentiate recombinants from the non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, galactosidase. This results into inactivation of the enzyme, which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the -galactosidase gene and the colonies do not produce any colour, these are identified as recombinant colonies.
Alternative selectable markers have been developed which differentiate recombinants from the non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, galactosidase. This results into inactivation of the enzyme, which is referred to as insertional inactivation. The presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. Presence of insert results into insertional inactivation of the -galactosidase gene and the colonies do not produce any colour, these are identified as recombinant colonies.
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In the process of insertional inactivationa)A recombinant DNA is inserted within the coding sequence of enzyme -galactosidase, resulting in inactivation of the enzymeb)A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmidc)A recombinat DNA is inserted within the recongnition site for EcoRld)None of the aboveCorrect answer is option 'A'. Can you explain this answer?
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In the process of insertional inactivationa)A recombinant DNA is inserted within the coding sequence of enzyme -galactosidase, resulting in inactivation of the enzymeb)A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmidc)A recombinat DNA is inserted within the recongnition site for EcoRld)None of the aboveCorrect answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about In the process of insertional inactivationa)A recombinant DNA is inserted within the coding sequence of enzyme -galactosidase, resulting in inactivation of the enzymeb)A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmidc)A recombinat DNA is inserted within the recongnition site for EcoRld)None of the aboveCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In the process of insertional inactivationa)A recombinant DNA is inserted within the coding sequence of enzyme -galactosidase, resulting in inactivation of the enzymeb)A recombinant DNA is inserted within the coding sequence of proteins involved in the replication of the plasmidc)A recombinat DNA is inserted within the recongnition site for EcoRld)None of the aboveCorrect answer is option 'A'. Can you explain this answer?.
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