Which of the following vectors has been used for introducing nematode...
Introduction:
In order to introduce nematode-specific genes in infected tobacco plants, different vectors have been used. Among them, Ti plasmid of Agrobacterium is the most commonly used vector.
Ti plasmid of Agrobacterium:
Agrobacterium tumefaciens is a soil bacterium that can transfer a part of its DNA (known as T-DNA) to the host plant. This process is called horizontal gene transfer and is facilitated by the presence of a plasmid called Ti (tumor-inducing) plasmid. The Ti plasmid contains genes responsible for the transfer of T-DNA to the plant genome.
Advantages of using Agrobacterium-mediated transformation:
1. High transformation efficiency
2. Precise integration of the foreign DNA into the plant genome
3. Stable inheritance of the transgene
4. Wide host range
5. No need for tissue culture
Nematode-specific genes:
Nematodes are a group of roundworms that can cause significant damage to plants. To combat nematode infestation, researchers have identified nematode-specific genes that can be introduced into the plant genome to confer resistance against nematodes.
Meloidogyne incognita:
Meloidogyne incognita is a species of root-knot nematode that can infect a wide range of plants, including tobacco. By introducing nematode-specific genes into tobacco plants using the Ti plasmid of Agrobacterium, researchers have been able to confer resistance against M. incognita.
Conclusion:
In summary, the Ti plasmid of Agrobacterium is a useful vector for introducing nematode-specific genes into infected tobacco plants. This approach has the potential to provide an effective and environmentally-friendly solution to nematode infestation in crops.
Which of the following vectors has been used for introducing nematode...
A tumor-inducing plasmid (Ti plasmid) is a plasmid found in pathogenic species of Agrobacterium. Ti plasmid contains a region called T-DNA which is replaced by our gene of interest (nematode specific genes) and Agrobacterium is then allowed to infect the tobacco plant. When Agrobacterium infects the tobacco plant, gene of interest (nematode specific genes) also gets inserted in tobacco.
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