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Which one of the following statement is not true regarding gel electrophoresis technique ?
- a)The separated DNA fragments are stained by using ethidium bromide.
- b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
- c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
- d)The process of extraction of separated DNA strands from gel is called elution.
Correct answer is option 'B'. Can you explain this answer?
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Which one of the following statement is not true regarding gel electro...
Explanation:
Gel electrophoresis is a commonly used technique in molecular biology to separate and analyze DNA fragments based on their size. It involves the migration of DNA fragments through a gel matrix under the influence of an electric field. While most of the statements mentioned in the options are true, option B is not true regarding the gel electrophoresis technique. Let's understand why:
Statement a: The separated DNA fragments are stained by using ethidium bromide.
Ethidium bromide is a commonly used fluorescent dye that intercalates between the base pairs of DNA. It binds to the DNA fragments present in the gel, allowing them to be visualized under UV light. This statement is true as the staining of DNA fragments with ethidium bromide is a common step in gel electrophoresis.
Statement b: The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
This statement is not true. Chromogenic substrates are usually used in other techniques such as enzyme-linked immunosorbent assay (ELISA) to generate a colorimetric response. In gel electrophoresis, the DNA fragments are not typically visualized using chromogenic substrates, but rather by using fluorescent dyes like ethidium bromide. Therefore, blue colored DNA bands due to the presence of a chromogenic substrate are not observed in gel electrophoresis.
Statement c: Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
This statement is true. After staining the DNA fragments with ethidium bromide, they can be visualized as bright orange bands when the gel is exposed to UV light. Ethidium bromide fluoresces under UV light, and the DNA fragments that have bound to the dye will appear as distinct bands.
Statement d: The process of extraction of separated DNA strands from gel is called elution.
This statement is true. Elution is the process of extracting the separated DNA strands from the gel matrix. It involves cutting out the desired DNA band from the gel and using various methods such as electroelution or using a gel extraction kit to separate the DNA from the gel and purify it for further analysis.
In conclusion, option B is not true because the presence of a chromogenic substrate does not give blue-colored DNA bands on the gel in gel electrophoresis.
Gel electrophoresis is a commonly used technique in molecular biology to separate and analyze DNA fragments based on their size. It involves the migration of DNA fragments through a gel matrix under the influence of an electric field. While most of the statements mentioned in the options are true, option B is not true regarding the gel electrophoresis technique. Let's understand why:
Statement a: The separated DNA fragments are stained by using ethidium bromide.
Ethidium bromide is a commonly used fluorescent dye that intercalates between the base pairs of DNA. It binds to the DNA fragments present in the gel, allowing them to be visualized under UV light. This statement is true as the staining of DNA fragments with ethidium bromide is a common step in gel electrophoresis.
Statement b: The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
This statement is not true. Chromogenic substrates are usually used in other techniques such as enzyme-linked immunosorbent assay (ELISA) to generate a colorimetric response. In gel electrophoresis, the DNA fragments are not typically visualized using chromogenic substrates, but rather by using fluorescent dyes like ethidium bromide. Therefore, blue colored DNA bands due to the presence of a chromogenic substrate are not observed in gel electrophoresis.
Statement c: Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
This statement is true. After staining the DNA fragments with ethidium bromide, they can be visualized as bright orange bands when the gel is exposed to UV light. Ethidium bromide fluoresces under UV light, and the DNA fragments that have bound to the dye will appear as distinct bands.
Statement d: The process of extraction of separated DNA strands from gel is called elution.
This statement is true. Elution is the process of extracting the separated DNA strands from the gel matrix. It involves cutting out the desired DNA band from the gel and using various methods such as electroelution or using a gel extraction kit to separate the DNA from the gel and purify it for further analysis.
In conclusion, option B is not true because the presence of a chromogenic substrate does not give blue-colored DNA bands on the gel in gel electrophoresis.
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Which one of the following statement is not true regarding gel electro...
In gel electrophoresis, the separated DNA fragments are typically visualized using a fluorescent dye, such as ethidium bromide, that intercalates with the DNA and fluoresces under UV light. This staining method gives a characteristic orange or red color to the DNA bands when exposed to UV light, not blue. Blue-colored DNA bands are not typically associated with the use of chromogenic substrates in gel electrophoresis.
Therefore, statement B is not true regarding the gel electrophoresis technique.
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Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer?
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Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer?.
Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer?.
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Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer?, a detailed solution for Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer? has been provided alongside types of Which one of the following statement is not true regarding gel electrophoresis technique ?a)The separated DNA fragments are stained by using ethidium bromide.b)The presence of chromogenic substrate gives blue coloured DNA bands on the gel.c)Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.d)The process of extraction of separated DNA strands from gel is called elution.Correct answer is option 'B'. Can you explain this answer? theory, EduRev gives you an
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