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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
- a)Easy availability of DNA template
- b)Availability of synthetic primers
- c)Availability of 'Thermostable' DNA polymerase
- d)Availability of cheap deoxyribonucleotides
Correct answer is option 'C'. Can you explain this answer?
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Which of the following contributed in popularising the PCR (polymerase...
Availability of thermostable dna polymerase made the rxn catalyed even at high temperature
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Which of the following contributed in popularising the PCR (polymerase...
Understanding PCR and Its Popularization
Polymerase Chain Reaction (PCR) is a revolutionary technique that allows for the amplification of DNA sequences, making it an essential tool in molecular biology, genetics, and medical diagnostics. Among the factors that contributed to its widespread adoption, the availability of thermostable DNA polymerase stands out as the most significant.
Why Thermostable DNA Polymerase is Key
- High Temperature Resilience: Traditional DNA polymerases are not stable at high temperatures required for PCR. The process involves repeated cycles of denaturation (separating DNA strands), annealing (binding primers), and extension (synthesizing new DNA strands). Thermostable polymerases, such as Taq polymerase, can withstand the high temperatures without denaturing, making the PCR process efficient and reliable.
- Increased Efficiency: The stability of these enzymes allows for rapid cycling through the PCR steps, leading to faster amplification of DNA. This efficiency is crucial when working with limited DNA samples, making PCR a go-to method for researchers.
- Wide Applicability: The robustness of thermostable DNA polymerases enables PCR to be utilized across various applications, including cloning, sequencing, and diagnostic testing. This versatility has led to the technique being adopted in numerous fields, from forensic science to clinical diagnostics.
In Summary
While other factors like the availability of synthetic primers and cheap deoxyribonucleotides are important, the introduction of thermostable DNA polymerases was the breakthrough that made PCR feasible and practical for widespread use. This advancement allowed scientists to amplify DNA efficiently and reliably, fundamentally transforming research and diagnostic approaches in molecular biology.
Polymerase Chain Reaction (PCR) is a revolutionary technique that allows for the amplification of DNA sequences, making it an essential tool in molecular biology, genetics, and medical diagnostics. Among the factors that contributed to its widespread adoption, the availability of thermostable DNA polymerase stands out as the most significant.
Why Thermostable DNA Polymerase is Key
- High Temperature Resilience: Traditional DNA polymerases are not stable at high temperatures required for PCR. The process involves repeated cycles of denaturation (separating DNA strands), annealing (binding primers), and extension (synthesizing new DNA strands). Thermostable polymerases, such as Taq polymerase, can withstand the high temperatures without denaturing, making the PCR process efficient and reliable.
- Increased Efficiency: The stability of these enzymes allows for rapid cycling through the PCR steps, leading to faster amplification of DNA. This efficiency is crucial when working with limited DNA samples, making PCR a go-to method for researchers.
- Wide Applicability: The robustness of thermostable DNA polymerases enables PCR to be utilized across various applications, including cloning, sequencing, and diagnostic testing. This versatility has led to the technique being adopted in numerous fields, from forensic science to clinical diagnostics.
In Summary
While other factors like the availability of synthetic primers and cheap deoxyribonucleotides are important, the introduction of thermostable DNA polymerases was the breakthrough that made PCR feasible and practical for widespread use. This advancement allowed scientists to amplify DNA efficiently and reliably, fundamentally transforming research and diagnostic approaches in molecular biology.
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?a)Easy availability of DNA templateb)Availability of synthetic primersc)Availability of Thermostable DNA polymerased)Availability of cheap deoxyribonucleotidesCorrect answer is option 'C'. Can you explain this answer?
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Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?a)Easy availability of DNA templateb)Availability of synthetic primersc)Availability of Thermostable DNA polymerased)Availability of cheap deoxyribonucleotidesCorrect answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?a)Easy availability of DNA templateb)Availability of synthetic primersc)Availability of Thermostable DNA polymerased)Availability of cheap deoxyribonucleotidesCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?a)Easy availability of DNA templateb)Availability of synthetic primersc)Availability of Thermostable DNA polymerased)Availability of cheap deoxyribonucleotidesCorrect answer is option 'C'. Can you explain this answer?.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?a)Easy availability of DNA templateb)Availability of synthetic primersc)Availability of Thermostable DNA polymerased)Availability of cheap deoxyribonucleotidesCorrect answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?a)Easy availability of DNA templateb)Availability of synthetic primersc)Availability of Thermostable DNA polymerased)Availability of cheap deoxyribonucleotidesCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?a)Easy availability of DNA templateb)Availability of synthetic primersc)Availability of Thermostable DNA polymerased)Availability of cheap deoxyribonucleotidesCorrect answer is option 'C'. Can you explain this answer?.
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