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Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be
(i) denatured double-stranded DNA probes.
(ii) double-stranded RNA probes.
(iii) protein probes.
(iv) single-stranded DNA probes
(i) denatured double-stranded DNA probes.
(ii) double-stranded RNA probes.
(iii) protein probes.
(iv) single-stranded DNA probes
- a)(i) and (ii)
- b)(ii) and (iii)
- c)(i)and(iv)
- d)(i), (ii), (iii) and (iv)
Correct answer is option 'C'. Can you explain this answer?
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Molecular probes used for identification of recombinant clone carrying...
The correct answer is option 'C' which includes (i) and (iv) molecular probes for the identification of recombinant clones carrying the desired DNA insert. Let's discuss each option in detail:
(i) Denatured double-stranded DNA probes:
Denatured double-stranded DNA probes are often used for the identification of recombinant clones. These probes are typically labeled with a radioactive or fluorescent tag and are complementary to the DNA sequence of interest. When these probes are added to a sample containing recombinant clones, they will hybridize specifically to the desired DNA insert, allowing for its detection. The denaturation step ensures that the DNA probes are single-stranded and can bind to the complementary sequence.
(iv) Single-stranded DNA probes:
Single-stranded DNA probes are another commonly used molecular probe for the identification of recombinant clones. These probes are also labeled with a radioactive or fluorescent tag and are designed to be complementary to the DNA sequence of interest. When added to a sample containing recombinant clones, the single-stranded DNA probe will hybridize specifically to the desired DNA insert, allowing for its detection.
(i) and (iv) are the correct options because both denatured double-stranded DNA probes and single-stranded DNA probes are widely used for the identification of recombinant clones carrying the desired DNA insert. These molecular probes provide a specific and sensitive method to detect the presence of the desired DNA sequence in the recombinant clones.
(ii) Double-stranded RNA probes:
Double-stranded RNA probes are not commonly used for the identification of recombinant clones. While RNA probes can be used for various applications, such as gene expression analysis, they are not typically used in the context of recombinant clone identification.
(iii) Protein probes:
Protein probes are also not commonly used for the identification of recombinant clones. Protein probes are often used for protein localization or interaction studies but are not typically used for DNA sequence detection.
In conclusion, the correct answer is option 'C' which includes (i) denatured double-stranded DNA probes and (iv) single-stranded DNA probes as the molecular probes used for the identification of recombinant clones carrying the desired DNA insert.
(i) Denatured double-stranded DNA probes:
Denatured double-stranded DNA probes are often used for the identification of recombinant clones. These probes are typically labeled with a radioactive or fluorescent tag and are complementary to the DNA sequence of interest. When these probes are added to a sample containing recombinant clones, they will hybridize specifically to the desired DNA insert, allowing for its detection. The denaturation step ensures that the DNA probes are single-stranded and can bind to the complementary sequence.
(iv) Single-stranded DNA probes:
Single-stranded DNA probes are another commonly used molecular probe for the identification of recombinant clones. These probes are also labeled with a radioactive or fluorescent tag and are designed to be complementary to the DNA sequence of interest. When added to a sample containing recombinant clones, the single-stranded DNA probe will hybridize specifically to the desired DNA insert, allowing for its detection.
(i) and (iv) are the correct options because both denatured double-stranded DNA probes and single-stranded DNA probes are widely used for the identification of recombinant clones carrying the desired DNA insert. These molecular probes provide a specific and sensitive method to detect the presence of the desired DNA sequence in the recombinant clones.
(ii) Double-stranded RNA probes:
Double-stranded RNA probes are not commonly used for the identification of recombinant clones. While RNA probes can be used for various applications, such as gene expression analysis, they are not typically used in the context of recombinant clone identification.
(iii) Protein probes:
Protein probes are also not commonly used for the identification of recombinant clones. Protein probes are often used for protein localization or interaction studies but are not typically used for DNA sequence detection.
In conclusion, the correct answer is option 'C' which includes (i) denatured double-stranded DNA probes and (iv) single-stranded DNA probes as the molecular probes used for the identification of recombinant clones carrying the desired DNA insert.
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Molecular probes used for identification of recombinant clone carrying...
Probe is a "single standard DNA molecule" that is complementary to gene of interest.so opt C is correct
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Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be(i) denatured double-stranded DNA probes.(ii) double-stranded RNA probes.(iii) protein probes.(iv) single-stranded DNA probesa)(i) and (ii)b)(ii) and (iii)c)(i)and(iv)d)(i), (ii), (iii) and (iv)Correct answer is option 'C'. Can you explain this answer?
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Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be(i) denatured double-stranded DNA probes.(ii) double-stranded RNA probes.(iii) protein probes.(iv) single-stranded DNA probesa)(i) and (ii)b)(ii) and (iii)c)(i)and(iv)d)(i), (ii), (iii) and (iv)Correct answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be(i) denatured double-stranded DNA probes.(ii) double-stranded RNA probes.(iii) protein probes.(iv) single-stranded DNA probesa)(i) and (ii)b)(ii) and (iii)c)(i)and(iv)d)(i), (ii), (iii) and (iv)Correct answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Molecular probes used for identification of recombinant clone carrying the desired DNA insert can be(i) denatured double-stranded DNA probes.(ii) double-stranded RNA probes.(iii) protein probes.(iv) single-stranded DNA probesa)(i) and (ii)b)(ii) and (iii)c)(i)and(iv)d)(i), (ii), (iii) and (iv)Correct answer is option 'C'. Can you explain this answer?.
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