Directions : In the following questions a statement of assertion (A) ...
EcoRI is a restriction endonuclease enzyme. Exonuclease removes nucleotides from the ends of DNA.
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Directions : In the following questions a statement of assertion (A) ...
Assertion (A) : EcoRI is a restriction endonuclease enzyme.
Reason (R) : Exonuclease removes nucleotides from the ends of DNA.
The correct answer is option B: Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).
Explanation:
EcoRI is indeed a restriction endonuclease enzyme. It is obtained from the bacterium Escherichia coli. EcoRI recognizes specific DNA sequences and cleaves the DNA at those sites, resulting in the formation of fragments with sticky ends. These sticky ends can then be used for various molecular biology techniques such as DNA cloning.
However, the reason provided is incorrect. Exonuclease is an enzyme that removes nucleotides from the ends of DNA, but it is not the reason why EcoRI is considered a restriction endonuclease. Restriction endonucleases, including EcoRI, cleave DNA at specific recognition sites within the DNA molecule, regardless of the presence or absence of nucleotides at the ends. The presence of exonuclease activity is not a defining characteristic of restriction endonucleases.
In summary, while assertion (A) is true, reason (R) is not the correct explanation for the assertion. EcoRI is a restriction endonuclease enzyme, but its classification as such is not based on the removal of nucleotides by exonucleases.
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