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Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :
Assertion (A) : It is essential to have few cloning sites in the cloning vector.
Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
- a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).
- b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).
- c)Assertion (A) is true but reason (R) is false.
- d)Assertion (A) is false but reason (R) is true.
Correct answer is option 'B'. Can you explain this answer?
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Directions : In the following questions a statement of assertion (A) ...
It is essential to have few cloning sites in the cloning vector. It is because, in order to link the alien DNA, the vector needs to have very few recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. Also, the vector requires a selectable marker, which helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.
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Directions : In the following questions a statement of assertion (A) ...
**Assertion (A) : It is essential to have few cloning sites in the cloning vector.**
**Reason (R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.**
The correct answer is option B, which states that both the assertion and reason are true, but the reason is not the correct explanation of the assertion. Let's discuss each statement in detail to understand why.
**Assertion (A) : It is essential to have few cloning sites in the cloning vector.**
Cloning sites are specific DNA sequences within a cloning vector where the DNA fragment of interest can be inserted. These sites typically contain recognition sequences for restriction enzymes, which can cut the DNA at specific points and allow the insertion of the desired DNA fragment. Having few cloning sites in the cloning vector is essential for several reasons:
1. Multiple Insertions: With few cloning sites, it becomes difficult to insert multiple DNA fragments into the vector simultaneously. This is important because if multiple fragments are inserted, it becomes challenging to identify and separate the desired transformants from non-transformants.
2. Screening: Having few cloning sites makes it easier to screen for successful transformations. After the insertion of the DNA fragment, the vector can be treated with restriction enzymes that do not cut within the cloning site. This will result in linearization of the vector if the DNA fragment has been successfully inserted. By screening for linearized vectors, non-transformants can be eliminated.
**Reason (R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.**
The reason provided is partially correct, but it does not fully explain why it is essential to have few cloning sites in the cloning vector. While it is true that having few cloning sites helps in identifying and eliminating non-transformants, it is not the only reason. The primary reason for having few cloning sites is to prevent multiple insertions and to facilitate the screening process.
In conclusion, both the assertion and reason are true, but the reason does not provide a complete explanation for why it is essential to have few cloning sites in the cloning vector.
**Reason (R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.**
The correct answer is option B, which states that both the assertion and reason are true, but the reason is not the correct explanation of the assertion. Let's discuss each statement in detail to understand why.
**Assertion (A) : It is essential to have few cloning sites in the cloning vector.**
Cloning sites are specific DNA sequences within a cloning vector where the DNA fragment of interest can be inserted. These sites typically contain recognition sequences for restriction enzymes, which can cut the DNA at specific points and allow the insertion of the desired DNA fragment. Having few cloning sites in the cloning vector is essential for several reasons:
1. Multiple Insertions: With few cloning sites, it becomes difficult to insert multiple DNA fragments into the vector simultaneously. This is important because if multiple fragments are inserted, it becomes challenging to identify and separate the desired transformants from non-transformants.
2. Screening: Having few cloning sites makes it easier to screen for successful transformations. After the insertion of the DNA fragment, the vector can be treated with restriction enzymes that do not cut within the cloning site. This will result in linearization of the vector if the DNA fragment has been successfully inserted. By screening for linearized vectors, non-transformants can be eliminated.
**Reason (R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.**
The reason provided is partially correct, but it does not fully explain why it is essential to have few cloning sites in the cloning vector. While it is true that having few cloning sites helps in identifying and eliminating non-transformants, it is not the only reason. The primary reason for having few cloning sites is to prevent multiple insertions and to facilitate the screening process.
In conclusion, both the assertion and reason are true, but the reason does not provide a complete explanation for why it is essential to have few cloning sites in the cloning vector.
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Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : It is essential to have few cloning sites in the cloning vector.Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'B'. Can you explain this answer?
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Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : It is essential to have few cloning sites in the cloning vector.Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : It is essential to have few cloning sites in the cloning vector.Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : It is essential to have few cloning sites in the cloning vector.Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'B'. Can you explain this answer?.
Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : It is essential to have few cloning sites in the cloning vector.Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : It is essential to have few cloning sites in the cloning vector.Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : It is essential to have few cloning sites in the cloning vector.Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'B'. Can you explain this answer?.
Solutions for Directions : In the following questions a statement of assertion (A) is followed by a statement of reason (R). Mark the correct choice as :Assertion (A) : It is essential to have few cloning sites in the cloning vector.Reason(R) : It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants.a)Both assertion (A) and reason (R) are true and reason (R) is the correct explanation of assertion (A).b)Both assertion (A) and reason (R) are true but reason (R) is not the correct explanation of assertion (A).c)Assertion (A) is true but reason (R) is false.d)Assertion (A) is false but reason (R) is true.Correct answer is option 'B'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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