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Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. 1. 'It' in the reason means cloning sites? 2. Are cloning site & Recognition sites same?
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Assertion (A): It is essential to have few cloning sites in cloning ve...
A cloning site refers to a specific DNA sequence in a cloning vector where a DNA fragment of interest can be inserted. This site is recognized by a specific enzyme, such as restriction endonuclease, which cuts the DNA at the cloning site.
To answer your questions:
1. Yes, in the reason, "it" refers to cloning sites. The cloning sites are essential because they provide the specific DNA sequences where the DNA fragment of interest can be inserted.
2. Cloning sites are not the same as transformants. Transformants are the cells or organisms that have taken up the recombinant DNA (containing the inserted DNA fragment) and are able to grow and replicate it. Cloning sites are necessary for the insertion of the DNA fragment into the cloning vector, but they do not directly determine the growth of transformants. However, having few cloning sites in a cloning vector can help in identifying and eliminating non-transformants because only transformants will have the inserted DNA fragment at the desired cloning site. In this way, the cloning sites can assist in selectively permitting the growth of the transformants.
To answer your questions:
1. Yes, in the reason, "it" refers to cloning sites. The cloning sites are essential because they provide the specific DNA sequences where the DNA fragment of interest can be inserted.
2. Cloning sites are not the same as transformants. Transformants are the cells or organisms that have taken up the recombinant DNA (containing the inserted DNA fragment) and are able to grow and replicate it. Cloning sites are necessary for the insertion of the DNA fragment into the cloning vector, but they do not directly determine the growth of transformants. However, having few cloning sites in a cloning vector can help in identifying and eliminating non-transformants because only transformants will have the inserted DNA fragment at the desired cloning site. In this way, the cloning sites can assist in selectively permitting the growth of the transformants.
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Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. 1. 'It' in the reason means cloning sites? 2. Are cloning site & Recognition sites same?
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Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. 1. 'It' in the reason means cloning sites? 2. Are cloning site & Recognition sites same? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. 1. 'It' in the reason means cloning sites? 2. Are cloning site & Recognition sites same? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. 1. 'It' in the reason means cloning sites? 2. Are cloning site & Recognition sites same?.
Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. 1. 'It' in the reason means cloning sites? 2. Are cloning site & Recognition sites same? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. 1. 'It' in the reason means cloning sites? 2. Are cloning site & Recognition sites same? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Assertion (A): It is essential to have few cloning sites in cloning vector. Reason(R): It helps in identifying and eliminating non-transformants and selectively permitting the growth of the transformants. 1. 'It' in the reason means cloning sites? 2. Are cloning site & Recognition sites same?.
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