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Choose the correct statement.
- a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.
- b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.
- c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.
- d)Rop helps in identification of transformed cells.
Correct answer is option 'A'. Can you explain this answer?
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Most Upvoted Answer
Choose the correct statement.a)Presence of more than one recognition ...
Explanation:
The correct statement is option A: Presence of more than one recognition site for one enzyme, within a vector, generates several fragments, which will complicate gene cloning.
Gene Cloning:
Gene cloning is the process of producing multiple copies of a specific gene or DNA fragment. It involves the insertion of a foreign DNA fragment into a vector, which is then replicated in a host organism.
Recognition Sites and Enzymes:
Enzymes called restriction enzymes or restriction endonucleases are used in gene cloning. These enzymes recognize specific DNA sequences, known as recognition sites, and cut the DNA at these sites. The recognition sites are typically palindromic, meaning they read the same forward and backward on complementary DNA strands.
Complication in Gene Cloning:
The presence of more than one recognition site for one enzyme within a vector can complicate gene cloning. When a vector contains multiple recognition sites, the enzyme will cut the DNA at each recognition site, resulting in the generation of several fragments.
Consequences of Multiple Fragments:
The generation of several fragments can lead to complications in gene cloning for the following reasons:
1. Fragment Length: The fragments generated may vary in size, making it difficult to isolate the desired DNA fragment.
2. Ligation: Ligation is the process of joining the foreign DNA fragment with the vector DNA. With multiple fragments, there is a higher chance of incorrect or incomplete ligation, resulting in the loss of the desired gene.
3. Transformants: Transformants are host cells that have successfully taken up the recombinant DNA. When multiple fragments are generated, it becomes challenging to identify the transformants containing the desired DNA fragment.
Conclusion:
In summary, the presence of more than one recognition site for one enzyme within a vector can lead to the generation of several fragments, complicating gene cloning. This can affect the isolation of the desired DNA fragment, the ligation process, and the identification of transformants. Therefore, option A is the correct statement.
The correct statement is option A: Presence of more than one recognition site for one enzyme, within a vector, generates several fragments, which will complicate gene cloning.
Gene Cloning:
Gene cloning is the process of producing multiple copies of a specific gene or DNA fragment. It involves the insertion of a foreign DNA fragment into a vector, which is then replicated in a host organism.
Recognition Sites and Enzymes:
Enzymes called restriction enzymes or restriction endonucleases are used in gene cloning. These enzymes recognize specific DNA sequences, known as recognition sites, and cut the DNA at these sites. The recognition sites are typically palindromic, meaning they read the same forward and backward on complementary DNA strands.
Complication in Gene Cloning:
The presence of more than one recognition site for one enzyme within a vector can complicate gene cloning. When a vector contains multiple recognition sites, the enzyme will cut the DNA at each recognition site, resulting in the generation of several fragments.
Consequences of Multiple Fragments:
The generation of several fragments can lead to complications in gene cloning for the following reasons:
1. Fragment Length: The fragments generated may vary in size, making it difficult to isolate the desired DNA fragment.
2. Ligation: Ligation is the process of joining the foreign DNA fragment with the vector DNA. With multiple fragments, there is a higher chance of incorrect or incomplete ligation, resulting in the loss of the desired gene.
3. Transformants: Transformants are host cells that have successfully taken up the recombinant DNA. When multiple fragments are generated, it becomes challenging to identify the transformants containing the desired DNA fragment.
Conclusion:
In summary, the presence of more than one recognition site for one enzyme within a vector can lead to the generation of several fragments, complicating gene cloning. This can affect the isolation of the desired DNA fragment, the ligation process, and the identification of transformants. Therefore, option A is the correct statement.
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Community Answer
Choose the correct statement.a)Presence of more than one recognition ...
In order to link the alien DNA, the vector needs to have very few, preferably single, recognition site for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
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Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer?
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Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer?.
Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer?.
Solutions for Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
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Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer?, a detailed solution for Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer? has been provided alongside types of Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice Choose the correct statement.a)Presence of more than one recognition site for one enzyme, within a vector, generate several fragments, which will complicate gene cloning.b)Ligation of alien DNA is carried out at a restriction site present in 'ori'.c)In pBR322, one antibiotic resistance gene helps in the selection of transformants, whereas rop helps in initiating replication.d)Rop helps in identification of transformed cells.Correct answer is option 'A'. Can you explain this answer? tests, examples and also practice NEET tests.
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