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If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, then
- a)Both transformed and untransformed recipient cell will die.
- b)Both transformed and untransformed cells will grow.
- c)Transformed recipient cell will grow and untransformed recipient cells will die.
- d)Transformed recipient cell will die and untransformed recipient cells will grow
Correct answer is option 'C'. Can you explain this answer?
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If a recombinant DNA bearing gene for ampicillin resistance is transf...
Explanation:
When a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, the following outcomes can be observed:
Transformed Recipient Cell:
- The transformed recipient cell refers to the E.coli cells that have successfully taken up the recombinant DNA containing the gene for ampicillin resistance.
- These cells will possess the ampicillin resistance gene and will be able to produce the enzyme beta-lactamase, which can degrade ampicillin and render it ineffective.
- As a result, these transformed recipient cells will be able to grow and survive on the agar plates containing ampicillin.
Untransformed Recipient Cells:
- The untransformed recipient cells refer to the E.coli cells that have not taken up the recombinant DNA and, therefore, do not possess the ampicillin resistance gene.
- These cells will not be able to produce the beta-lactamase enzyme and, thus, will not be able to degrade ampicillin.
- As a result, these untransformed recipient cells will not be able to grow and survive on the agar plates containing ampicillin.
Outcome:
- Based on the above explanations, the correct answer is option 'C' - the transformed recipient cells will grow and the untransformed recipient cells will die.
- This is because only the transformed recipient cells possess the ampicillin resistance gene and can produce the beta-lactamase enzyme to degrade ampicillin, allowing them to survive on the agar plates. The untransformed recipient cells, lacking the ampicillin resistance gene, cannot survive in the presence of ampicillin and will die.
In Summary:
- The transformation of E.coli cells with a recombinant DNA bearing the gene for ampicillin resistance allows the transformed recipient cells to grow and survive on agar plates containing ampicillin.
- On the other hand, the untransformed recipient cells, which do not possess the ampicillin resistance gene, cannot degrade ampicillin and will die in the presence of this antibiotic.
- This selective growth of transformed recipient cells and death of untransformed recipient cells on agar plates containing ampicillin is a fundamental technique used in molecular biology to identify and isolate cells that have successfully taken up recombinant DNA.
When a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, the following outcomes can be observed:
Transformed Recipient Cell:
- The transformed recipient cell refers to the E.coli cells that have successfully taken up the recombinant DNA containing the gene for ampicillin resistance.
- These cells will possess the ampicillin resistance gene and will be able to produce the enzyme beta-lactamase, which can degrade ampicillin and render it ineffective.
- As a result, these transformed recipient cells will be able to grow and survive on the agar plates containing ampicillin.
Untransformed Recipient Cells:
- The untransformed recipient cells refer to the E.coli cells that have not taken up the recombinant DNA and, therefore, do not possess the ampicillin resistance gene.
- These cells will not be able to produce the beta-lactamase enzyme and, thus, will not be able to degrade ampicillin.
- As a result, these untransformed recipient cells will not be able to grow and survive on the agar plates containing ampicillin.
Outcome:
- Based on the above explanations, the correct answer is option 'C' - the transformed recipient cells will grow and the untransformed recipient cells will die.
- This is because only the transformed recipient cells possess the ampicillin resistance gene and can produce the beta-lactamase enzyme to degrade ampicillin, allowing them to survive on the agar plates. The untransformed recipient cells, lacking the ampicillin resistance gene, cannot survive in the presence of ampicillin and will die.
In Summary:
- The transformation of E.coli cells with a recombinant DNA bearing the gene for ampicillin resistance allows the transformed recipient cells to grow and survive on agar plates containing ampicillin.
- On the other hand, the untransformed recipient cells, which do not possess the ampicillin resistance gene, cannot degrade ampicillin and will die in the presence of this antibiotic.
- This selective growth of transformed recipient cells and death of untransformed recipient cells on agar plates containing ampicillin is a fundamental technique used in molecular biology to identify and isolate cells that have successfully taken up recombinant DNA.
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If a recombinant DNA bearing gene for ampicillin resistance is transf...
If a recombinant DNA-bearing gene for resistance to an antibiotic (ampicillin) is transferred into E.coli cells, the host cells become transformed into ampicillin-resistant cells. If we spread the transformed cells on agar plates containing ampicillin, only transformants will grow, untransformed recipient cells will die. The ampicillin resistance gene, in this case, is called a selectable marker.
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If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, thena)Both transformed and untransformed recipient cell will die.b)Both transformed and untransformed cells will grow.c)Transformed recipient cell will grow and untransformed recipient cells will die.d)Transformed recipient cell will die and untransformed recipient cells will growCorrect answer is option 'C'. Can you explain this answer?
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If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, thena)Both transformed and untransformed recipient cell will die.b)Both transformed and untransformed cells will grow.c)Transformed recipient cell will grow and untransformed recipient cells will die.d)Transformed recipient cell will die and untransformed recipient cells will growCorrect answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, thena)Both transformed and untransformed recipient cell will die.b)Both transformed and untransformed cells will grow.c)Transformed recipient cell will grow and untransformed recipient cells will die.d)Transformed recipient cell will die and untransformed recipient cells will growCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, thena)Both transformed and untransformed recipient cell will die.b)Both transformed and untransformed cells will grow.c)Transformed recipient cell will grow and untransformed recipient cells will die.d)Transformed recipient cell will die and untransformed recipient cells will growCorrect answer is option 'C'. Can you explain this answer?.
If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, thena)Both transformed and untransformed recipient cell will die.b)Both transformed and untransformed cells will grow.c)Transformed recipient cell will grow and untransformed recipient cells will die.d)Transformed recipient cell will die and untransformed recipient cells will growCorrect answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, thena)Both transformed and untransformed recipient cell will die.b)Both transformed and untransformed cells will grow.c)Transformed recipient cell will grow and untransformed recipient cells will die.d)Transformed recipient cell will die and untransformed recipient cells will growCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, thena)Both transformed and untransformed recipient cell will die.b)Both transformed and untransformed cells will grow.c)Transformed recipient cell will grow and untransformed recipient cells will die.d)Transformed recipient cell will die and untransformed recipient cells will growCorrect answer is option 'C'. Can you explain this answer?.
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ample number of questions to practice If a recombinant DNA bearing gene for ampicillin resistance is transferred into E.coli cells and the host cells are spread on agar plates containing ampicillin, thena)Both transformed and untransformed recipient cell will die.b)Both transformed and untransformed cells will grow.c)Transformed recipient cell will grow and untransformed recipient cells will die.d)Transformed recipient cell will die and untransformed recipient cells will growCorrect answer is option 'C'. Can you explain this answer? tests, examples and also practice NEET tests.
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