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What is the purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology?
- a)To enhance DNA ligation
- b)To amplify the gene of interest
- c)To select transformed cells
- d)To extract the desired product
Correct answer is option 'C'. Can you explain this answer?
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What is the purpose of using a selectable marker gene like ampicillin ...
Selectable marker genes like ampicillin resistance help in identifying and selecting cells that have taken up the recombinant DNA.
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What is the purpose of using a selectable marker gene like ampicillin ...
Introduction:
In recombinant DNA technology, selectable marker genes are used to distinguish transformed cells from non-transformed cells. These marker genes provide a selective advantage to the cells that have taken up the recombinant DNA, allowing for their identification and isolation.
Explanation:
Selectable marker genes, such as ampicillin resistance, are commonly used in recombinant DNA technology for the following reasons:
1. Selective advantage:
Selectable marker genes provide a selective advantage to the transformed cells that have successfully incorporated the recombinant DNA. In the case of ampicillin resistance, the gene encodes for an enzyme called β-lactamase, which confers resistance to the antibiotic ampicillin. Therefore, only the cells that have taken up the recombinant DNA containing the selectable marker gene will be able to grow and survive in the presence of ampicillin.
2. Selection of transformed cells:
By including the selectable marker gene in the recombinant DNA construct, researchers can selectively grow and isolate only those cells that have successfully taken up the desired gene or DNA fragment of interest. This allows for the identification and selection of transformed cells from a population of non-transformed cells.
3. Identification of successful transformation:
The presence of the selectable marker gene, such as ampicillin resistance, allows researchers to easily identify successful transformations. Transformed cells will be able to grow on selective media containing ampicillin, while non-transformed cells will not survive. This simplifies the screening process and confirms the presence of the desired gene or DNA fragment in the transformed cells.
Conclusion:
The purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology is to provide a selective advantage to the transformed cells, allowing for their identification and isolation. This marker gene helps in distinguishing transformed cells from non-transformed cells and facilitates the selection of cells that have successfully taken up the desired gene or DNA fragment of interest.
In recombinant DNA technology, selectable marker genes are used to distinguish transformed cells from non-transformed cells. These marker genes provide a selective advantage to the cells that have taken up the recombinant DNA, allowing for their identification and isolation.
Explanation:
Selectable marker genes, such as ampicillin resistance, are commonly used in recombinant DNA technology for the following reasons:
1. Selective advantage:
Selectable marker genes provide a selective advantage to the transformed cells that have successfully incorporated the recombinant DNA. In the case of ampicillin resistance, the gene encodes for an enzyme called β-lactamase, which confers resistance to the antibiotic ampicillin. Therefore, only the cells that have taken up the recombinant DNA containing the selectable marker gene will be able to grow and survive in the presence of ampicillin.
2. Selection of transformed cells:
By including the selectable marker gene in the recombinant DNA construct, researchers can selectively grow and isolate only those cells that have successfully taken up the desired gene or DNA fragment of interest. This allows for the identification and selection of transformed cells from a population of non-transformed cells.
3. Identification of successful transformation:
The presence of the selectable marker gene, such as ampicillin resistance, allows researchers to easily identify successful transformations. Transformed cells will be able to grow on selective media containing ampicillin, while non-transformed cells will not survive. This simplifies the screening process and confirms the presence of the desired gene or DNA fragment in the transformed cells.
Conclusion:
The purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology is to provide a selective advantage to the transformed cells, allowing for their identification and isolation. This marker gene helps in distinguishing transformed cells from non-transformed cells and facilitates the selection of cells that have successfully taken up the desired gene or DNA fragment of interest.
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What is the purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology?a)To enhance DNA ligationb)To amplify the gene of interestc)To select transformed cellsd)To extract the desired productCorrect answer is option 'C'. Can you explain this answer?
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What is the purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology?a)To enhance DNA ligationb)To amplify the gene of interestc)To select transformed cellsd)To extract the desired productCorrect answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about What is the purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology?a)To enhance DNA ligationb)To amplify the gene of interestc)To select transformed cellsd)To extract the desired productCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for What is the purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology?a)To enhance DNA ligationb)To amplify the gene of interestc)To select transformed cellsd)To extract the desired productCorrect answer is option 'C'. Can you explain this answer?.
What is the purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology?a)To enhance DNA ligationb)To amplify the gene of interestc)To select transformed cellsd)To extract the desired productCorrect answer is option 'C'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about What is the purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology?a)To enhance DNA ligationb)To amplify the gene of interestc)To select transformed cellsd)To extract the desired productCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for What is the purpose of using a selectable marker gene like ampicillin resistance in recombinant DNA technology?a)To enhance DNA ligationb)To amplify the gene of interestc)To select transformed cellsd)To extract the desired productCorrect answer is option 'C'. Can you explain this answer?.
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