NEET Exam > NEET Questions > Plasmid pBR322 has PstI restriction enzyme si...
Start Learning for Free
Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli strain
- a)it will not be able to confer ampicillin resistance to the host cell.
- b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.
- c)it will lead to lysis of host cell.
- d)it will be able to produce a novel protein with dual ability.
Correct answer is option 'A'. Can you explain this answer?
| FREE This question is part of | Download PDF Attempt this Test |
Most Upvoted Answer
Plasmid pBR322 has PstI restriction enzyme site within gene αmpR...
Plasmid pBR322 is a commonly used cloning vector in molecular biology. It contains several unique restriction enzyme sites that allow for the insertion of foreign DNA fragments.
One of the restriction enzyme sites present in pBR322 is the PstI site. PstI is a type II restriction enzyme that recognizes the DNA sequence 5'-CTGCAG-3' and cuts between the C and T nucleotides.
In pBR322, the PstI site is located within one of the genes present in the plasmid. This means that if the PstI enzyme is used to digest the plasmid DNA, it will cleave the DNA at the PstI site within the gene.
This can be useful in molecular biology experiments, as it allows for the removal or replacement of the gene with a different DNA fragment of interest. After digestion with PstI, the plasmid can be ligated with a foreign DNA fragment that has also been digested with PstI, resulting in the insertion of the foreign DNA into the plasmid.
Overall, the presence of a PstI site within a gene in pBR322 provides a convenient way to manipulate the plasmid and introduce desired genetic material.
One of the restriction enzyme sites present in pBR322 is the PstI site. PstI is a type II restriction enzyme that recognizes the DNA sequence 5'-CTGCAG-3' and cuts between the C and T nucleotides.
In pBR322, the PstI site is located within one of the genes present in the plasmid. This means that if the PstI enzyme is used to digest the plasmid DNA, it will cleave the DNA at the PstI site within the gene.
This can be useful in molecular biology experiments, as it allows for the removal or replacement of the gene with a different DNA fragment of interest. After digestion with PstI, the plasmid can be ligated with a foreign DNA fragment that has also been digested with PstI, resulting in the insertion of the foreign DNA into the plasmid.
Overall, the presence of a PstI site within a gene in pBR322 provides a convenient way to manipulate the plasmid and introduce desired genetic material.
Free Test
FREE
| Start Free Test |
Community Answer
Plasmid pBR322 has PstI restriction enzyme site within gene αmpR...
pBR322 has two antibiotic-resistant genes for ampicillin (ampR) and tetracycline (tetR). Due to the insertion of the gene in the ampR region, insertional inactivation results in the inactivation of the ampicillin gene and hence the organisms become sensitive to this antibiotic and do not grow in the media containing this antibiotic.
Attention NEET Students!
To make sure you are not studying endlessly, EduRev has designed NEET study material, with Structured Courses, Videos, & Test Series. Plus get personalized analysis, doubt solving and improvement plans to achieve a great score in NEET.
|
Explore Courses for NEET exam
|
|
Similar NEET Doubts
Top Courses for NEETView all
Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer?
Question Description
Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer?.
Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer?.
Solutions for Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? in English & in Hindi are available as part of our courses for NEET.
Download more important topics, notes, lectures and mock test series for NEET Exam by signing up for free.
Here you can find the meaning of Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of
Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer?, a detailed solution for Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? has been provided alongside types of Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice Plasmid pBR322 has PstI restriction enzyme site within gene αmpR that confers ampicillin resistance. If this enzyme is used for inserting a gene f or β-galactoside production and the recombinant plasmid is inserted in an E . coli straina)it will not be able to confer ampicillin resistance to the host cell.b)the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.c)it will lead to lysis of host cell.d)it will be able to produce a novel protein with dual ability.Correct answer is option 'A'. Can you explain this answer? tests, examples and also practice NEET tests.
|
|
Explore Courses for NEET exam
|
|
Suggested Free Tests
Signup for Free!
Signup to see your scores go up within 7 days! Learn & Practice with 1000+ FREE Notes, Videos & Tests.
|
© EduRev
|
Education Revolution
|
Follow Us
|
Signup to see your scores
go up within 7 days!
Access 1000+ FREE Docs, Videos and Tests
Takes less than 10 seconds to signup