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Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain
  • a)
    It will not be able to confer ampicillin resistance to the host cell
  • b)
    The transformed cells will have the ability to resist ampicillin as well as produce β-galactoside
  • c)
    It will lead to lysis of host cell
  • d)
    It will be able to produce a novel protein with dual ability
Correct answer is option 'A'. Can you explain this answer?
Most Upvoted Answer
Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that...
PBR322 is a commonly used cloning vector. When the gene for β-galactoside is inserted in the ampicillin resistance gene by using Pst I, the recombinant E.coli will lose ampicillin resistance due to insertional inactivation of the antibiotic resistance gene.
The host (recombinant) cell will produce β-galactoside which is not a novel protein nor does it have dual ability.
The transformed cells cannot resist ampicillin as they have lost ampicillin resistance.
A recombinant E. coli is produced and the host cell will not undergo lysis due to insertion of β-galactoside gene.
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Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli straina)It will not be able to confer ampicillin resistance to the host cellb)The transformed cells will have the ability to resist ampicillin as well as produce β-galactosidec)It will lead to lysis of host celld)It will be able to produce a novel protein with dual abilityCorrect answer is option 'A'. Can you explain this answer?
Question Description
Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli straina)It will not be able to confer ampicillin resistance to the host cellb)The transformed cells will have the ability to resist ampicillin as well as produce β-galactosidec)It will lead to lysis of host celld)It will be able to produce a novel protein with dual abilityCorrect answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli straina)It will not be able to confer ampicillin resistance to the host cellb)The transformed cells will have the ability to resist ampicillin as well as produce β-galactosidec)It will lead to lysis of host celld)It will be able to produce a novel protein with dual abilityCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Plasmid pBR322 has Pstl restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli straina)It will not be able to confer ampicillin resistance to the host cellb)The transformed cells will have the ability to resist ampicillin as well as produce β-galactosidec)It will lead to lysis of host celld)It will be able to produce a novel protein with dual abilityCorrect answer is option 'A'. Can you explain this answer?.
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