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If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-
- a)Show ampicillin resistance only
- b)Show tetracycline resistance
- c)Will grow well on tetracycline containing medium
- d)Will not grow on ampicillin containing medium
Correct answer is option 'A'. Can you explain this answer?
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Most Upvoted Answer
If we ligate a foreign DNA at the Bam H1 site of tetracycline resistan...
Explanation:
Recombinant plasmids are formed by the ligation of foreign DNA into the cloning site of a vector. In this case, the foreign DNA is being ligated into the Bam H1 site of the tetracycline resistance gene in pBR322.
Ampicillin Resistance:
The pBR322 vector contains an ampicillin resistance gene. Therefore, the recombinant plasmid will also show ampicillin resistance. This is because the ampicillin resistance gene is not affected by the ligation of foreign DNA.
Tetracycline Resistance:
The Bam H1 site of the tetracycline resistance gene is the site where the tetracycline resistance gene is cut during the ligation process. Therefore, the recombinant plasmid will not show tetracycline resistance. This is because the tetracycline resistance gene has been disrupted by the ligation process.
Growth on Tetracycline Containing Medium:
The recombinant plasmid will not grow on tetracycline-containing medium. This is because the tetracycline resistance gene has been disrupted by the ligation process.
Growth on Ampicillin Containing Medium:
The recombinant plasmid will grow on ampicillin-containing medium. This is because the ampicillin resistance gene is not affected by the ligation process.
Conclusion:
In conclusion, the recombinant plasmid will show ampicillin resistance only and will grow on ampicillin-containing medium, but will not show tetracycline resistance and will not grow on tetracycline-containing medium.
Recombinant plasmids are formed by the ligation of foreign DNA into the cloning site of a vector. In this case, the foreign DNA is being ligated into the Bam H1 site of the tetracycline resistance gene in pBR322.
Ampicillin Resistance:
The pBR322 vector contains an ampicillin resistance gene. Therefore, the recombinant plasmid will also show ampicillin resistance. This is because the ampicillin resistance gene is not affected by the ligation of foreign DNA.
Tetracycline Resistance:
The Bam H1 site of the tetracycline resistance gene is the site where the tetracycline resistance gene is cut during the ligation process. Therefore, the recombinant plasmid will not show tetracycline resistance. This is because the tetracycline resistance gene has been disrupted by the ligation process.
Growth on Tetracycline Containing Medium:
The recombinant plasmid will not grow on tetracycline-containing medium. This is because the tetracycline resistance gene has been disrupted by the ligation process.
Growth on Ampicillin Containing Medium:
The recombinant plasmid will grow on ampicillin-containing medium. This is because the ampicillin resistance gene is not affected by the ligation process.
Conclusion:
In conclusion, the recombinant plasmid will show ampicillin resistance only and will grow on ampicillin-containing medium, but will not show tetracycline resistance and will not grow on tetracycline-containing medium.
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Community Answer
If we ligate a foreign DNA at the Bam H1 site of tetracycline resistan...
A is the correct answer. since a foreign DNA was ligated at the place of tetracycline gene, it has lost the property of resistant to tetracycline and only ampicillin gene is present.Hence it is resistant only to ampicillin
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If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer?
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If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer?.
If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer?.
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If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer?, a detailed solution for If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer? has been provided alongside types of If we ligate a foreign DNA at the Bam H1 site of tetracycline resistance gene in pBR322 the recombinant plasmid will-a)Show ampicillin resistance only b)Show tetracycline resistancec)Will grow well on tetracycline containing mediumd)Will not grow on ampicillin containing mediumCorrect answer is option 'A'. Can you explain this answer? theory, EduRev gives you an
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