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A selectable marker is used to :
- a)Mark a gene on a chromosome for isolation using restriction enzyme.
- b)Help in eliminating the non-transformants, so that the transformants can be regenerated.
- c)Identify the gene for a desired trait in an alien organism.
- d)Select a suitable vector for transformation in a specific crop.
Correct answer is option 'B'. Can you explain this answer?
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Most Upvoted Answer
A selectable marker is used to :a)Mark a gene on a chromosome for iso...
Explanation:
A selectable marker is a gene that is introduced into a cell or organism during genetic engineering experiments. It is used to distinguish between cells that have been successfully transformed with a desired gene and those that have not. The correct answer to the given question is option 'B'. A selectable marker helps in eliminating the non-transformants so that the transformants can be regenerated.
Benefits of using a selectable marker:
Using a selectable marker in genetic engineering experiments has several benefits, including:
- Identification of transformed cells: A selectable marker allows researchers to easily identify and select cells that have successfully taken up the desired gene. This is crucial in determining the success of the transformation experiment.
- Elimination of non-transformed cells: By using a selectable marker, non-transformed cells can be eliminated from the population, ensuring that only the desired transformed cells are used for further analysis or experimentation.
- Regeneration of transformed cells: After eliminating the non-transformed cells, the transformed cells can be regenerated and grown in culture. This allows for the production of a large population of cells that carry the desired gene.
- Selection of appropriate vectors: Selectable markers can also be used to select suitable vectors for transformation in specific crops. Different selectable markers can be used to determine the most effective vector for transformation.
- Efficient gene transfer: Selectable markers are also helpful in ensuring efficient gene transfer by selecting only those cells that have successfully taken up the desired gene.
Conclusion:
Selectable markers are an important tool in genetic engineering experiments as they allow for the identification and selection of cells that have successfully taken up a desired gene. By eliminating non-transformed cells and regenerating the transformed cells, selectable markers ensure the production of a population of cells that carry the desired gene. They also help in selecting suitable vectors for transformation in specific crops.
A selectable marker is a gene that is introduced into a cell or organism during genetic engineering experiments. It is used to distinguish between cells that have been successfully transformed with a desired gene and those that have not. The correct answer to the given question is option 'B'. A selectable marker helps in eliminating the non-transformants so that the transformants can be regenerated.
Benefits of using a selectable marker:
Using a selectable marker in genetic engineering experiments has several benefits, including:
- Identification of transformed cells: A selectable marker allows researchers to easily identify and select cells that have successfully taken up the desired gene. This is crucial in determining the success of the transformation experiment.
- Elimination of non-transformed cells: By using a selectable marker, non-transformed cells can be eliminated from the population, ensuring that only the desired transformed cells are used for further analysis or experimentation.
- Regeneration of transformed cells: After eliminating the non-transformed cells, the transformed cells can be regenerated and grown in culture. This allows for the production of a large population of cells that carry the desired gene.
- Selection of appropriate vectors: Selectable markers can also be used to select suitable vectors for transformation in specific crops. Different selectable markers can be used to determine the most effective vector for transformation.
- Efficient gene transfer: Selectable markers are also helpful in ensuring efficient gene transfer by selecting only those cells that have successfully taken up the desired gene.
Conclusion:
Selectable markers are an important tool in genetic engineering experiments as they allow for the identification and selection of cells that have successfully taken up a desired gene. By eliminating non-transformed cells and regenerating the transformed cells, selectable markers ensure the production of a population of cells that carry the desired gene. They also help in selecting suitable vectors for transformation in specific crops.
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A selectable marker is used to :a)Mark a gene on a chromosome for iso...
Selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture that confers a trait suitable for artificial selection.
Selectable markers help in identification and elimination of non-transformants whilst permitting selective growth of transformants.
Note: Selectable marker genes are conditionally dominant genes that confer an ability to grow in the presence of applied selective agents that are normally toxic to plant cells or inhibitory to plant growth, such as antibiotics and herbicides. Genes that are frequently used to select transformed plant tissues include nptII, hpt, bar, and gox, that confer resistance to kanamycin, hygromycin, phosphinothricin,and glyphosate, respectively. Commonly used selectable marker systems include neomycin phosphotransferase, hygromycin phosphotransferase, phosphoinothricin acetyltransferase, and glyphosate oxidoreductase.
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A selectable marker is used to :a)Mark a gene on a chromosome for isolation using restriction enzyme.b)Help in eliminating the non-transformants, so that the transformants can be regenerated.c)Identify the gene for a desired trait in an alien organism.d)Select a suitable vector for transformation in a specific crop.Correct answer is option 'B'. Can you explain this answer?
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A selectable marker is used to :a)Mark a gene on a chromosome for isolation using restriction enzyme.b)Help in eliminating the non-transformants, so that the transformants can be regenerated.c)Identify the gene for a desired trait in an alien organism.d)Select a suitable vector for transformation in a specific crop.Correct answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about A selectable marker is used to :a)Mark a gene on a chromosome for isolation using restriction enzyme.b)Help in eliminating the non-transformants, so that the transformants can be regenerated.c)Identify the gene for a desired trait in an alien organism.d)Select a suitable vector for transformation in a specific crop.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A selectable marker is used to :a)Mark a gene on a chromosome for isolation using restriction enzyme.b)Help in eliminating the non-transformants, so that the transformants can be regenerated.c)Identify the gene for a desired trait in an alien organism.d)Select a suitable vector for transformation in a specific crop.Correct answer is option 'B'. Can you explain this answer?.
A selectable marker is used to :a)Mark a gene on a chromosome for isolation using restriction enzyme.b)Help in eliminating the non-transformants, so that the transformants can be regenerated.c)Identify the gene for a desired trait in an alien organism.d)Select a suitable vector for transformation in a specific crop.Correct answer is option 'B'. Can you explain this answer? for NEET 2024 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about A selectable marker is used to :a)Mark a gene on a chromosome for isolation using restriction enzyme.b)Help in eliminating the non-transformants, so that the transformants can be regenerated.c)Identify the gene for a desired trait in an alien organism.d)Select a suitable vector for transformation in a specific crop.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for NEET 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A selectable marker is used to :a)Mark a gene on a chromosome for isolation using restriction enzyme.b)Help in eliminating the non-transformants, so that the transformants can be regenerated.c)Identify the gene for a desired trait in an alien organism.d)Select a suitable vector for transformation in a specific crop.Correct answer is option 'B'. Can you explain this answer?.
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