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All questions of Genetic Technology for A Level Exam

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The component which is not required in PCR is__.
  • a)
    Mg2+
  • b)
    Polymerase
  • c)
    Calcium ions
  • d)
    dNTPs
Correct answer is option 'C'. Can you explain this answer?

Gaurav Kumar answered
  • The polymerase chain reaction is a technology in molecular biology used to amplify a single copy or a few copies of a piece of DNA.
  • Calcium ions is not required in PCR technology.

Which one is called molecular scissors?
a) Endocrine
b) Ribonuclease
c) Restriction enzymes
d) Exonuclease
Correct answer is option 'C'. Can you explain this answer?

Lavanya Menon answered
Restriction enzymes are also called 'molecular scissors' as they cleave DNA at or near specific recognition sequences known as restriction sites. These enzymes make one incision on each of the two strands of DNA and are also called restriction endonucleases.

The group of letters that form same words when read both forward and backward are called?
  • a)
    Palindrome
  • b)
    Puzzle
  • c)
    Endonucleases
  • d)
    Sticky ends
Correct answer is option 'A'. Can you explain this answer?

Pooja Saha answered
The groups of letters that form same words when read both forward and backward are called Palindrome. For example MALYALAM which read same from both side.

Having two antibiotic resistant genes in the same plasmid
  • a)
    two antibiotics add to the effect for selection
  • b)
    helps in identifying transformants
  • c)
    one antibiotic does not gives the sufficient result
  • d)
    one antibiotic may not be functional
Correct answer is option 'B'. Can you explain this answer?

Pranjal Shah answered
Explanation:
Plasmids are circular pieces of DNA that exist outside of the bacterial chromosome, and they can carry genes that provide specific advantages to the bacteria in certain conditions. One such advantage is antibiotic resistance, which allows the bacteria to survive in the presence of antibiotics.

When scientists want to introduce a new gene into bacteria, they often use plasmids as vectors to deliver the gene. This is called transformation, and it can be a useful tool for genetic engineering.

Having two antibiotic resistant genes in the same plasmid can be helpful in identifying transformants, which are bacteria that have successfully taken up the plasmid and incorporated the new gene into their own DNA.

Here are some reasons why having two antibiotic resistant genes in the same plasmid can be useful:

- Selection: If the plasmid carries two different antibiotic resistant genes, then scientists can use two different antibiotics to select for bacteria that have taken up the plasmid. This increases the chances of finding transformants, because the bacteria that are resistant to both antibiotics are more likely to have the plasmid with the new gene.

- Identification: If the plasmid carries two different antibiotic resistant genes, then scientists can use a combination of both antibiotics to identify the transformants. This is because only bacteria that have taken up the plasmid will be resistant to both antibiotics, and this makes it easier to distinguish them from other bacteria in the culture.

- Functionality: If the plasmid carries two different antibiotic resistant genes, then it is less likely that one of the antibiotics will be ineffective. This is because some bacteria can acquire resistance to a single antibiotic through a mutation or other mechanism, but it is less likely that they will acquire resistance to two different antibiotics at the same time.

Overall, having two antibiotic resistant genes in the same plasmid can be a useful tool for genetic engineering, because it helps to increase the efficiency and accuracy of the transformation process.

Insertional inactivation helps in
  • a)
    X-gal breakdown
  • b)
    Identification of recombinant clones
  • c)
    Ampicillin resistance
  • d)
    Functioning of B-galactosidase
Correct answer is option 'B'. Can you explain this answer?

Insertional inactivation is a technique used in molecular biology to identify recombinant clones. It involves the insertion of a foreign DNA fragment into a plasmid vector, which disrupts the function of a selectable marker gene. This results in the loss of the ability of the host cell to grow on a selective medium containing the corresponding antibiotic.

Mechanism

The mechanism of insertional inactivation involves the insertion of a foreign DNA fragment into a plasmid vector, which disrupts the function of a selectable marker gene. The selectable marker gene is usually a gene that confers resistance to an antibiotic, such as ampicillin. The disruption of this gene results in the loss of the ability of the host cell to grow on a selective medium containing the corresponding antibiotic. This allows for the identification of recombinant clones that have taken up the plasmid vector with the foreign DNA fragment.

Applications

Insertional inactivation is commonly used in molecular biology to identify recombinant clones. It is particularly useful in the construction of cDNA libraries and the screening of genomic DNA libraries for specific genes. It can also be used to study gene expression and regulation, as well as for the production of recombinant proteins.

Conclusion

In conclusion, insertional inactivation is a useful technique in molecular biology for identifying recombinant clones. It involves the insertion of a foreign DNA fragment into a plasmid vector, which disrupts the function of a selectable marker gene. This results in the loss of the ability of the host cell to grow on a selective medium containing the corresponding antibiotic, allowing for the identification of recombinant clones that have taken up the plasmid vector with the foreign DNA fragment.

Enzymes used to join foreign DNA to plasmid is______.
  • a)
    Nucleases
  • b)
    Ligases
  • c)
    Endonucleases
  • d)
    Pectinases
Correct answer is option 'B'. Can you explain this answer?

The Enzymes used to join foreign DNA to plasmid is ligases. The plasmid of bacteria replicate this DNA strands along with other which can be further transferred to target cells.

 A technology which has found immense use in solving cases of disputed parentage is
  • a)
    Monoclonal antibody production
  • b)
    DNA finger printing
  • c)
    Polymerase chain reaction
  • d)
    Recombinant DNA technology
Correct answer is option 'B'. Can you explain this answer?

Mohammad Karim answered
Option B - for exapmple for identifying the true father DNA samples of child, mother and possible fathers are taken and their DNA fingerprints are obtained. the print of child DNA Match to the prints of biological parents

A ladder is used in Gel electrophoresis as it helps in_____.
  • a)
    it helps in denaturation of DNA
  • b)
    it helps in EtBr binding
  • c)
    Comparing the size of the DNA fragment
  • d)
    It prevents the movement of DNA out of the gel
Correct answer is option 'C'. Can you explain this answer?

A molecular-weight size marker, also referred to as a protein ladder, DNA ladder, or RNA ladder, is a set of standards that are used to identify the approximate size of a molecule run on a gel during electrophoresis.

The American company which is involved in the manufacturing of Humulin is
  • a)
    Elixir
  • b)
    Ranbaxy
  • c)
    Prohealth
  • d)
    Eli Lily
Correct answer is option 'D'. Can you explain this answer?

Harshitha Dey answered
In 1983, the American company Eli Lily prepared two DNA sequences corresponding to A and B and introduced into plasmid of E.coli to produce insulin chain.

Conversion of sugar into alcohol during fermentation is due to the direct action of
  • a)
    Temperature
  • b)
    Microorganisms
  • c)
    Zymase
  • d)
    Concentration of sugar solution
Correct answer is option 'C'. Can you explain this answer?

Zymase is the enzyme which changes the simple sugars, dextrose and fructose into carbon dioxide gas and alcohol at 370C, and several esters which result in producing the unique aroma flavor and taste of bread.
Sugar or starch is used as the carbohydrate source. Zymase is the enzyme found in yeast and some bacteria.

* that why option """c""" is correct

The smallest bands in the agarose gel will be towards
  • a)
    Wells
  • b)
    Middle of the gel
  • c)
    Cathode
  • d)
    Anode
Correct answer is option 'D'. Can you explain this answer?

Sonal Kulkarni answered
Gel electrophoresis technique involves separation of different segments of DNA according to their size. The smallest bands in the agarose gel will be towards positively charged anode.

Rop genes in pBR322 codes for______.
  • a)
    Tetracycline resistance
  • b)
    Antibiotic resistance
  • c)
    Proteins involved in replication of plasmid
  • d)
    Ampicillin resistance
Correct answer is option 'C'. Can you explain this answer?

Yash Saha answered
Rop genes in pBR322 codes for protein involved in replication of plasmid. Plasmid are able to take the foreign gene and to be transferred to target cells.

Which of the following is not applicable for a biopatent?
  • a)
    Formation of new products
  • b)
    Unearthing the DNA sequences
  • c)
    Discovery of cell lines
  • d)
    Using the existing technology to produce new products
Correct answer is option 'C'. Can you explain this answer?

Geetika Shah answered
A biological patent is a patent on an invention in the field of biology that by law allows the patent holder to exclude others from making, using, selling, or importing the protected invention for a limited period of time. Hence, the correct option is c.

The term ‘Humulin’ is used for
  • a)
    Hydrolytic enzyme
  • b)
    Isoenzyme
  • c)
    Human insulin
  • d)
    Antibiotic
Correct answer is option 'C'. Can you explain this answer?

Rohan Singh answered
Humulin is human insulin used for treating diabetes. Prior to its development, diabetics used insulin isolated from pig and cow pancreases. Developed by Genentech, the first American biotechnology company, Humulin was licensed to Eli Lilly and became the first marketable product created through recombinant DNA technology. Its licensing by the FDA in October 1982 also made it the first recombinant pharmaceutical approved for use in the United States.

Which organelle is wrongly paired?
  • a)
    Mitochondria-Kreb’s cycle
  • b)
    Plastid-Photosynthesis
  • c)
    RER-proteins
  • d)
    Plasmid-denaturation
Correct answer is option 'D'. Can you explain this answer?

Gaurav Kumar answered
  • Plasmids renature because they have supercoiled structures that have held the two strands of the helix together during denaturation.
  • Chromosomal DNA is not able to renature, however, because its longer strands have become mixed with denatured proteins.

The particles used to coat with DNA in Biolistic gun is of
  • a)
    Tungsten
  • b)
    Zinc
  • c)
    Helium
  • d)
    Quartz
Correct answer is option 'A'. Can you explain this answer?

DNA is coated with tungsten before used in biolistic gun for inserting the DNA directly into target cells for obtaining particular protein.

 Cultivation of Bt cotton has been much in the news. The prefix Bt means
  • a)
    “Barium treated” cotton seeds
  • b)
    “Bigger thread” variety of cotton with better tensile strength
  • c)
    Carrying an endotoxin gene from Bacillus thuringiensis
  • d)
    Produced by “biotechnology” using restriction enzymes and ligases
Correct answer is option 'C'. Can you explain this answer?

Rajeev Saxena answered
Bt-cotton, Bt-tobacco, Bt-tomato etc., are transgenic plants having Bt-2 gene encoding Bt toxin (e.g., Thurioside). Bt-toxin gene has been isolated from a bacteria Bacillus thuringiensis there for called Bt. (i.e., Bacillus thuringiensis). These plants are resistant for more than 140 species of insects including common cabbage worm, tent caterpillars, bag worms, canker worms, gypsy worm etc.

Denaturation is done at high temperature as it leads to
  • a)
    Breaking of hydrogen bonds between the bases
  • b)
    Termination of the reaction
  • c)
    Polymerization
  • d)
    Joining of primers at the 5’ end
Correct answer is option 'A'. Can you explain this answer?

Ananya Das answered
  • All the enzymes at higher temperature become inactive.
  • Denaturation is done at high temperature as it leads to breaking down of hydrogen bonds between the bases of polynucleotides.

Father of green revolution is
  • a)
    Karl Landsteiner
  • b)
    Vurghese Kurein
  • c)
    Haberlandt
  • d)
    M.S. Swaminathan
Correct answer is option 'D'. Can you explain this answer?

Syed Hussain answered
MS Swaminathan, known as the 'Father of Green Revolution' was born on August 7, 1925. Swaminathandeveloped high-yielding varieties (HYV) of wheat and later, promoted sustainable development which he called, the 'evergreen revolution.

The nuclease will act on______.
  • a)
    DNA only
  • b)
    Proteins
  • c)
    RNA only
  • d)
    DNA and RNA
Correct answer is option 'D'. Can you explain this answer?

Arya Khanna answered
The enzyme nuclease acts on nucleic acid DNA and RNA. This enzyme is used to hydrolyze nucleic acid.

A bioreactor is:
  • a)
    Culture for synthesis of new chemicals
  • b)
    Hybridoma
  • c)
    Culture containing radioactive isotopes
  • d)
    Fermentation tank
Correct answer is option 'D'. Can you explain this answer?

Krishna Iyer answered
Fermentation is defined more from the point of view of engineers. They see fermentation as the cultivation of high amount of microorganisms and biotransformation being carried out in special vessels called fermenter or bioreactors.

Hybridoma technology has been successfully used in
  • a)
    Production of alcohol in bulk
  • b)
    Synthesis of monoclonal antibodies
  • c)
    Synthesis of haemoglobin
  • d)
    Production of somatic hybrids
Correct answer is option 'B'. Can you explain this answer?

Rajat Kapoor answered
Hybridoma technology has contributed to virtually all areas of biology and medicine and set the scene for important advances in cell biology and immunodiagnostics. Blood transfusion and transfusion medicine have benefited significantly from development in hybridoma technology, and this has resulted in the generation of a wide range of monoclonal antibodies to human blood group antigens. These antibodies have also had an impact on diagnostic techniques such as enzyme-linked immunosorbent assay and flow cytometry as well as on the application of therapeutic agents in the clinical environment. However, rapid advances in the development of knockout and transgenic mice as well as in nucleic acid-based immunization may well lead hybridoma technology into a new era.

The term antibiotic was coined by
  • a)
    Alexander Fleming
  • b)
    S.Waksman
  • c)
    Louis Pasteur
  • d)
    Edward Jenner
Correct answer is option 'B'. Can you explain this answer?

Sanjana Singh answered
Selman Waksman, the microbiologist who discovered streptomycin , first used the word 'Antibiotic' in medical in 1943.
So, the Correct answers is 'Waksman'.

Biotechnology has advantages over conventional breeding as
  • a)
    Growth rate is less
  • b)
    It is less time consuming
  • c)
    Growth rate is more but milk production is less
  • d)
    It is selective breeding
Correct answer is option 'B'. Can you explain this answer?

Rajeev Saxena answered
Genetic engineering is not just an extension of conventional breeding. In fact, it differs profoundly. As a general rule, conventional breeding develops new plant varieties by the process of selection, and seeks to achieve expression of genetic material which is already present within a species.

A transgenic food crop which may help in solving the problem of night blindness in developing countries is
  • a)
    Bt soybean
  • b)
    Flavrsavr tomatoes
  • c)
    Golden rice
  • d)
    Starlink maize
Correct answer is option 'C'. Can you explain this answer?

Chirag Ghosh answered
Golden Rice as a solution to night blindness

Golden Rice is a genetically modified rice variety that has been developed to help combat vitamin A deficiency, which can lead to night blindness and other health problems in developing countries. The rice is genetically modified to produce beta-carotene, a precursor to vitamin A, which is not naturally found in traditional rice varieties.

How does Golden Rice work?

Golden Rice is genetically engineered to produce beta-carotene in the endosperm of the rice grain, which is the part of the plant that is eaten. Beta-carotene is a pigment that gives fruits and vegetables their orange or yellow color, and it is also a precursor to vitamin A. When humans consume beta-carotene, it is converted to vitamin A in the body.

Why is Golden Rice important?

Vitamin A deficiency is a serious health problem in many developing countries, particularly in Southeast Asia and Africa. According to the World Health Organization, an estimated 250 million preschool children are vitamin A deficient, and it is estimated that between 250,000 and 500,000 malnourished children become blind every year due to vitamin A deficiency. Golden Rice is one potential solution to this problem, as it provides a source of beta-carotene that can be converted to vitamin A in the body.

Conclusion

Golden Rice is a promising solution to the problem of vitamin A deficiency in developing countries, which can lead to night blindness and other health problems. While there is still some controversy surrounding genetically modified crops, Golden Rice has the potential to make a significant impact on the health and well-being of millions of people around the world.

Which one of the microorganism is used for production of citric acid in industries ?
  • a)
    Lactobacillus bulgaricus
  • b)
    Penicillium citrinum
  • c)
    Aspergillus niger
  • d)
    Rhizopus nigricans
Correct answer is option 'C'. Can you explain this answer?

Vivek Patel answered
Microorganisms can produce citric acid. The fungus Aspergillus Niger is most commonly used for industrial production of citric acid. The other organisms (although less important) include A. clavatus, A. wentii, Penicillium luteum, Candida catenula, C. guilliermondii and Corynebacterium sp.

 Insect resistance transgenic cotton has been produced by inserting a piece of DNA from
  • a)
    A virus
  • b)
    A bacterium
  • c)
    A wild relative of cotton
  • d)
    An insect
Correct answer is option 'B'. Can you explain this answer?

Syed Saif answered
Bacillus killing the larvae of flour moths in Germany and published a description of the bacterium and its … Mycogen, another small biotechnology firm, instead cloned Bt genes and expressed them in an alternate host, Pseudomonas fluorescens, a bacterial species common in transgenic cotton.

Which out of the following is advantage of chemical safety testing on transgenic animals?
  • a)
    Testing can be performed easily
  • b)
    Testing is very cost effective
  • c)
    Obtain results in less time
  • d)
    All of the above
Correct answer is option 'C'. Can you explain this answer?

Dipika Das answered
Transgenic animals are made to carry gene that make them sensitive to toxic substance than non-transgenic animals and exposed to toxic substance. Toxicity testing in transgenic animals allows us to obtain results in less time.

Phage is a______.
  • a)
    Fungi
  • b)
    Virion
  • c)
    Viroids
  • d)
    Virus that infects bacteria
Correct answer is option 'D'. Can you explain this answer?

Phage is a virus that infects bacteria. Such viruses transfer their genetic materials into host bacteria and using the bacterial machinery increase in number and multiply rapidly to kill host cells.

The bacteria generally used for genetic engineering is                              
  • a)
    Agro bacterium
  • b)
    Bacillus
  • c)
    Pseudomonas
  • d)
    Clostridium
Correct answer is option 'A'. Can you explain this answer?

Ananya Singh answered
The two most important bacterias are E.coli (which is used for commercial production) and Agrobacterium i.e. agrobacterium tumifaciens.
Agrobacterium has T. DNA which is transferred in host cells. That's why this bacteria is called as natural genetic engineer which is generally used for genetic engineering.

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